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通过依赖于血管内皮钙黏蛋白的转胞吞作用进行细胞成分的细胞间交换。

Inter-cellular exchange of cellular components via VE-cadherin-dependent trans-endocytosis.

作者信息

Sakurai Takashi, Woolls Melissa J, Jin Suk-Won, Murakami Masahiro, Simons Michael

机构信息

Yale Cardiovascular Research Center and Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America.

Yale Cardiovascular Research Center and Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, United States of America; Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, United States of America.

出版信息

PLoS One. 2014 Mar 6;9(6):e90736. doi: 10.1371/journal.pone.0090736. eCollection 2014.

DOI:10.1371/journal.pone.0090736
PMID:24603875
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3946293/
Abstract

Cell-cell communications typically involve receptor-mediated signaling initiated by soluble or cell-bound ligands. Here, we report a unique mode of endocytosis: proteins originating from cell-cell junctions and cytosolic cellular components from the neighboring cell are internalized, leading to direct exchange of cellular components between two adjacent endothelial cells. VE-cadherins form transcellular bridges between two endothelial cells that are the basis of adherence junctions. At such adherens junction sites, we observed the movement of the entire VE-cadherin molecule from one endothelial cell into the other with junctional and cytoplasmic components. This phenomenon, here termed trans-endocytosis, requires the establishment of a VE-cadherin homodimer in trans with internalization proceeding in a Rac1-, and actomyosin-dependent manner. Importantly, the trans-endocytosis is not dependent on any known endocytic pathway including clathrin-dependent endocytosis, macropinocytosis or phagocytosis. This novel form of cell-cell communications, leading to a direct exchange of cellular components, was observed in 2D and 3D-cultured endothelial cells as well as in the developing zebrafish vasculature.

摘要

细胞间通讯通常涉及由可溶性或细胞结合配体引发的受体介导信号传导。在此，我们报告一种独特的内吞作用模式：源自细胞间连接的蛋白质和来自相邻细胞的胞质细胞成分被内化，导致两个相邻内皮细胞之间直接进行细胞成分交换。血管内皮钙黏蛋白在两个内皮细胞之间形成跨细胞桥，这是黏附连接的基础。在这种黏附连接位点，我们观察到整个血管内皮钙黏蛋白分子从一个内皮细胞进入另一个内皮细胞，并伴有连接和细胞质成分。这种现象，在此称为跨内吞作用，需要建立反式血管内皮钙黏蛋白同型二聚体，内化过程以Rac1和肌动球蛋白依赖的方式进行。重要的是，跨内吞作用不依赖于任何已知的内吞途径，包括网格蛋白依赖的内吞作用、巨胞饮作用或吞噬作用。这种导致细胞成分直接交换的新型细胞间通讯形式，在二维和三维培养的内皮细胞以及发育中的斑马鱼脉管系统中均有观察到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/51fb/3946293/974e4501ee82/pone.0090736.g008.jpg
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