Wang Rui, Li Zhi, Guo Hui, Shi Wei, Xin Yue, Chang Weilong, Huang Tao
Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
Mol Med Rep. 2014 May;9(5):1723-8. doi: 10.3892/mmr.2014.2018. Epub 2014 Mar 6.
Previous studies have demonstrated that caveolin 1 acts as a tumor suppressor in breast cancer, however, few studies have demonstrated that caveolin 1 also serves as a tumor promoter in breast cancer. In the present study, caveolin 1 small interfering RNA was used to knock down caveolin 1 expression in order to investigate the association between caveolin 1 and the proliferation and metastatic abilities of human breast cancer BT474 cells. The results revealed that cell proliferation, migration and invasion were attenuated by caveolin 1 knockdown in BT474 cells. Furthermore, caveolin 1 knockdown in BT474 cells arrested cells in the G0/G1 phase and decreased the number of cells in the S phase. In addition, caveolin 1 knockdown decreased the activation of the extracellular signal-regulated kinase 1/2 pathway and inhibited the expression of cell cycle-associated proteins (cyclin D1, c-Fos and β-catenin), whilst the expression of E-cadherin was increased. Furthermore, the protein expression of matrix metalloproteinase-2, -9 and -1 was also inhibited by caveolin 1 knockdown. In combination, these results demonstrated that caveolin 1 knockdown had a tumor suppressing effect on BT474 cells.
先前的研究表明，小窝蛋白1在乳腺癌中起肿瘤抑制作用，然而，很少有研究表明小窝蛋白1在乳腺癌中也可作为肿瘤促进因子。在本研究中，使用小窝蛋白1小干扰RNA敲低小窝蛋白1的表达，以研究小窝蛋白1与人类乳腺癌BT474细胞增殖和转移能力之间的关联。结果显示，敲低BT474细胞中的小窝蛋白1可减弱细胞增殖、迁移和侵袭。此外，敲低BT474细胞中的小窝蛋白1会使细胞停滞在G0/G1期，并减少S期细胞数量。此外，敲低小窝蛋白1会降低细胞外信号调节激酶1/2途径的激活，并抑制细胞周期相关蛋白（细胞周期蛋白D1、c-Fos和β-连环蛋白）的表达，同时E-钙黏蛋白的表达增加。此外，敲低小窝蛋白1还会抑制基质金属蛋白酶-2、-9和-1的蛋白表达。综合来看，这些结果表明敲低小窝蛋白1对BT474细胞具有肿瘤抑制作用。
