Itoh K, Sasaki R, Ono K, Tezuka H, Sakoda H, Sawada H, Hitomi K, Nakane H, Uchiyama T, Uchino H
Department of Biology, Faculty of Science, Niigata University.
Jpn J Cancer Res. 1988 Aug;79(8):931-7. doi: 10.1111/j.1349-7006.1988.tb00057.x.
Transplantable erythroblastic leukemia was induced by 300-rad irradiation of C3H mice. Conditions for in vitro growth of the leukemic cells were studied. None of interleukin-3, granulocyte/macrophage colony-stimulating factor and erythropoietin could support the growth of the cells in vitro. In contrast, the leukemic cells grew into a stroma-dependent cell line, ELM-D, in close contact with the stromal cell layer of 900-rad-irradiated long-term bone marrow culture. A stroma-independent cell line, termed ELM-I-1, was further established from the non-adherent population in the co-culture of the leukemic cells, ELM-D, with stromal cells. Reverse transcriptase activity was not detectable in ELM-D or ELM-I-1 cells. Studies on binding and cross-linking of 125I-erythropoietin showed that ELM-I-1 cells had erythropoietin receptors, and two major radiolabeled protein products with molecular weights of 120 kDa and 140 kDa were detected on sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions.
通过对C3H小鼠进行300拉德的辐射诱导出可移植性成红细胞白血病。对白血病细胞的体外生长条件进行了研究。白细胞介素-3、粒细胞/巨噬细胞集落刺激因子和促红细胞生成素均不能支持细胞的体外生长。相反，白血病细胞与经900拉德辐射的长期骨髓培养的基质细胞层紧密接触，生长成为一种依赖基质的细胞系ELM-D。从白血病细胞ELM-D与基质细胞共培养的非贴壁群体中进一步建立了一种不依赖基质的细胞系，称为ELM-I-1。在ELM-D或ELM-I-1细胞中未检测到逆转录酶活性。对125I-促红细胞生成素的结合和交联研究表明，ELM-I-1细胞具有促红细胞生成素受体，在还原条件下的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳上检测到两种主要的放射性标记蛋白产物，分子量分别为120 kDa和140 kDa。
