Department of Astrocyte Biology and Neurodegeneration, The Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands Academy of Sciences, Amsterdam, The Netherlands; Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida, USA; Department of Neuropathology, Academic Medical Center, Amsterdam, The Netherlands; Swammerdam Institute for Life Sciences, Center for Neuroscience, University of Amsterdam, Amsterdam, The Netherlands; Department of Translational Neuroscience, Brain Center Rudolf Magnus, University Medical Center, Utrecht, The Netherlands.
Stem Cells Transl Med. 2014 Apr;3(4):470-80. doi: 10.5966/sctm.2013-0038. Epub 2014 Mar 6.
Neural progenitor cells (NPCs) in the subventricular zone (SVZ) hold promise for future therapy for neurodegenerative disorders, because the stimulation of adult neurogenesis could potentially restore the function of degenerating neurons and glia. To obtain more knowledge on these NPCs, we developed a method to specifically isolate NPCs from postmortem adult human brains based on the expression of the specific human adult neural stem/progenitor cell marker glial fibrillary acidic protein δ (GFAPδ). An extensive immunophenotyping analysis for cell surface markers resulted in the observation that CD271 was limited to the SVZ-derived GFAPδ-positive cells. CD271(+) cells developed into neurospheres and could be differentiated into astrocytes, neurons, and oligodendrocytes. We are the first to show that a pure population of NPCs can be isolated from the adult human SVZ, which is highly instrumental for developing future therapies based on stimulating endogenous SVZ neurogenesis.
神经祖细胞（NPCs）在侧脑室下区（SVZ）具有治疗神经退行性疾病的潜力，因为刺激成年神经发生可能会恢复退化神经元和神经胶质的功能。为了获得更多关于这些 NPCs 的知识，我们开发了一种基于特定的人类成年神经干细胞/祖细胞标志物胶质纤维酸性蛋白 δ（GFAPδ）表达的方法，专门从死后成人脑中分离 NPCs。对细胞表面标志物的广泛免疫表型分析表明，CD271 仅限于 SVZ 衍生的 GFAPδ 阳性细胞。CD271(+)细胞可形成神经球，并能分化为星形胶质细胞、神经元和少突胶质细胞。我们首次表明，可以从成人 SVZ 中分离出纯 NPC 群体，这对于开发基于刺激内源性 SVZ 神经发生的未来治疗方法非常重要。
