通过原位杂交检测组织和细胞中的目的基因。

Detection of genes of interest in tissues and cells by in situ hybridization.

作者信息

Seibel N L, Smith-Gill S, Hollis G F, Kirsch I R

机构信息

National Cancer Institute, Navy Medical Oncology Branch, Bethesda, Maryland 20814.

出版信息

J Virol Methods. 1988 Sep;21(1-4):171-7. doi: 10.1016/0166-0934(88)90063-8.

DOI:10.1016/0166-0934(88)90063-8

PMID:2460481

Abstract

In situ hybridization (ISH) is a powerful technique for assessing the expression of particular genes of interest within individual cells in tissues or cytospins. The only limitations of the technique are the availability of appropriate probes and tissues. When coupled with routine histology and DNA analysis, characterization and classification of tissues can be greatly extended. Future applications include the identification of tumor-specific markers that can be combined with ISH in diagnostic strategies.

摘要

原位杂交（ISH）是一种用于评估组织或细胞涂片单个细胞内特定感兴趣基因表达的强大技术。该技术的唯一局限性在于合适探针和组织的可获得性。当与常规组织学和DNA分析相结合时，组织的特征描述和分类可以得到极大扩展。未来的应用包括鉴定可在诊断策略中与ISH相结合的肿瘤特异性标志物。