Seibel N L, Funa K, Dmitrovsky E, Foss F, Hollis G F, Kirsch I R
Hum Pathol. 1987 Jan;18(1):3-8. doi: 10.1016/s0046-8177(87)80186-7.
RNA-RNA tissue in situ hybridization is a relatively new technique that detects gene expression in individual cells. In this report we compare and contrast the technique with conventional biologic analysis. We illustrate how this technique could function as a diagnostic tool by applying it to a 58-year-old man with a four-month history of lymphadenopathy and peripheral lymphocytosis. RNA-RNA tissue in situ hybridization performed on sections of one of this patient's lymph nodes and on cytospins of his peripheral blood demonstrated the presence of an apparent monoclonal population of B cells producing mu and lambda immunoglobulin (Ig) messages in the lymph node and peripheral blood as well as a T-cell population in the lymph node only. These results were corroborative and complementary to conventional DNA (Southern) and RNA (Northern) analyses. The data were consistent with the diagnosis of chronic lymphocytic leukemia (CLL). With the use of this technique, an intriguing pattern of cellular heterogeneity was observed within the mu-lambda population of cells in the lymph node. A subset of these cells appeared to express a much greater amount of immunoglobulin message and to cluster around the lymph node vessels. The combination of RNA-RNA in situ hybridization and routine histopathology has the potential for providing an additional dimension to tumor analysis.
RNA-RNA组织原位杂交是一种相对较新的技术，可检测单个细胞中的基因表达。在本报告中，我们将该技术与传统生物学分析进行比较和对比。我们通过将其应用于一名有四个月淋巴结病和外周淋巴细胞增多病史的58岁男性，来说明该技术如何作为一种诊断工具发挥作用。对该患者的一个淋巴结切片和外周血细胞涂片进行RNA-RNA组织原位杂交，结果显示在淋巴结和外周血中存在明显的产生μ和λ免疫球蛋白（Ig）信息的B细胞单克隆群体，并且仅在淋巴结中存在T细胞群体。这些结果与传统的DNA（Southern）和RNA（Northern）分析相互印证且互为补充。数据与慢性淋巴细胞白血病（CLL）的诊断一致。使用该技术时，在淋巴结的μ-λ细胞群体中观察到了有趣的细胞异质性模式。这些细胞的一个亚群似乎表达了大量的免疫球蛋白信息，并聚集在淋巴结血管周围。RNA-RNA原位杂交与常规组织病理学相结合，有可能为肿瘤分析提供一个新的维度。
