六种市售单克隆免疫荧光试剂用于直接诊断沙眼衣原体感染的染色特性。
Staining characteristics of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections.
作者信息
Cles L D, Bruch K, Stamm W E
机构信息
Department of Medicine, School of Medicine, University of Washington, Seattle 98195.
出版信息
J Clin Microbiol. 1988 Sep;26(9):1735-7. doi: 10.1128/jcm.26.9.1735-1737.1988.
Abstract
Using purified elementary bodies of 14 Chlamydia trachomatis serovars in an in vitro assay, we compared the staining characteristics of six commercially available monoclonal antibody reagents used for direct immunofluorescent staining of patient smears. Considerable variation in the degree of brightness, consistency of staining, and specificity of the six reagents was found. Monoclonal antibodies against the major outer membrane proteins of C. trachomatis produced brighter fluorescence, more consistent elementary body morphology, and less nonspecific staining than did monoclonal antibodies directed against chlamydial lipopolysaccharide.
摘要
在一项体外试验中，我们使用14种沙眼衣原体血清型的纯化原体，比较了用于患者涂片直接免疫荧光染色的六种市售单克隆抗体试剂的染色特性。发现这六种试剂在亮度、染色一致性和特异性方面存在相当大的差异。与针对衣原体脂多糖的单克隆抗体相比，针对沙眼衣原体主要外膜蛋白的单克隆抗体产生的荧光更亮，原体形态更一致，非特异性染色更少。
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