Taylor M A, Ferrell R V, Wise K S, McIntosh M A
Department of Microbiology, School of Medicine, University of Missouri, Columbia 65212.
Mol Microbiol. 1988 Sep;2(5):665-72. doi: 10.1111/j.1365-2958.1988.tb00075.x.
Genomic restriction fragments isolated from Mycoplasma hyorhinis and Mycoplasma hyopneumoniae were shown by DNA hybridization and nucleotide sequence analyses to contain sequences common to these two species, as well as another porcine-derived mycoplasma, Mycoplasma flocculare. Intraspecies hybridization experiments using these fragments as probes indicated that the sequence is highly redundant in several strains of M. hyorhinis, but that there is diversity in the sizes of restriction fragments detected among these strains. In contrast, repetition of the sequence was limited in M. hyopneumoniae and M. flocculare, and no homologies to this repeated element were apparent in mycoplasma species isolated from animal hosts other than the swine. The reiterated sequence may reflect intraspecies genomic diversification in M. hyorhinis and its selective presence in otherwise unrelated species raises the possibility that it has been horizontally transmitted between these organisms.
通过DNA杂交和核苷酸序列分析表明，从猪鼻支原体和猪肺炎支原体中分离出的基因组限制性片段包含这两个物种以及另一种源自猪的支原体——絮状支原体共有的序列。使用这些片段作为探针进行种内杂交实验表明，该序列在猪鼻支原体的几个菌株中高度冗余，但这些菌株中检测到的限制性片段大小存在差异。相比之下，该序列在猪肺炎支原体和絮状支原体中的重复有限，并且在从猪以外的动物宿主分离出的支原体物种中未发现与该重复元件的同源性。这种重复序列可能反映了猪鼻支原体的种内基因组多样化，其在其他不相关物种中的选择性存在增加了它在这些生物体之间水平传播的可能性。
