Johansson K E, Mattsson J G, Jacobsson K, Fernandez C, Bergström K, Bölske G, Wallgren P, Göbel U B
National Veterinary Institute, Laboratory of Bacteriology, Uppsala, Sweden.
Res Vet Sci. 1992 Mar;52(2):195-204. doi: 10.1016/0034-5288(92)90010-y.
Mycoplasma is the common name for the smallest free-living microorganisms, the Mollicutes. Mycoplasma hyopneumoniae is of great importance in veterinary medicine, causing enzootic pneumonia in pigs. M hyorhinis can cause polyserositis and may cause pneumonia in piglets. Oligonucleotides complementary to variable regions of 16S rRNA from these mycoplasmas were designed and used as probes for detection and identification of these mycoplasmas. The probe complementary to 16S rRNA of M hyorhinis gave a very weak cross-hybridisation with M hyosynoviae in filter hybridisation experiments, but not with any of the other porcine mycoplasmas tested. Three oligonucleotide probes complementary to M hyopneumoniae 16S rRNA were tested. One of the probes (Mhp6/30) was found to be specific to M hyopneumoniae, but the other two gave cross-hybridisation with M flocculare. Using the Mhp6/30 probe in direct filter hybridisation experiments, it proved possible to detect M hyopneumoniae in lung biopsies from experimentally infected pigs.
支原体是最小的自由生活微生物——柔膜菌纲的通用名称。猪肺炎支原体在兽医学中非常重要，可引起猪的地方性肺炎。猪鼻支原体可导致多浆膜炎，并可能导致仔猪肺炎。设计了与这些支原体16S rRNA可变区互补的寡核苷酸，并将其用作检测和鉴定这些支原体的探针。在滤膜杂交实验中，与猪鼻支原体16S rRNA互补的探针与猪滑膜支原体有非常微弱的交叉杂交，但与测试的其他任何猪支原体均无交叉杂交。测试了三种与猪肺炎支原体16S rRNA互补的寡核苷酸探针。其中一种探针（Mhp6/30）被发现对猪肺炎支原体具有特异性，但另外两种与絮状支原体有交叉杂交。在直接滤膜杂交实验中使用Mhp6/30探针，证明可以从实验感染猪的肺活检组织中检测到猪肺炎支原体。
