Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691-4096, USA.
Bioresour Technol. 2014 Apr;158:248-52. doi: 10.1016/j.biortech.2014.02.029. Epub 2014 Feb 17.
Fungal pretreatment is an environmentally friendly process that has been widely studied to improve the digestibility of lignocellulosic biomass. However, sterilization of feedstocks, a costly process, is generally required prior to the fungal pretreatment. In this study, fungal pretreatment of unsterilized yard trimmings using yard trimmings pre-colonized with Ceriporiopsis subvermispora as an inoculum was investigated. Degradation of lignin, cellulose, hemicellulose, and dry matter in yard trimmings during 30 days of fungal pretreatment using different inoculum/substrate ratios (1:19, 1:9 and 1:4) was 14.8-20.2%, 8.1-15.4%, 20.7-27.8%, and 9.8-16.2%, respectively. Methane yields of 34.9-44.6L/kg volatile solids were achieved during solid-state anaerobic digestion (SS-AD) of the pretreated yard trimmings, which were comparable to those obtained by using the traditional method requiring feedstock sterilization. The technology developed in this study can save about 501-789 kJ/kg of dry yard trimmings processed, which is about half of the total biogas energy produced by SS-AD.
真菌预处理是一种环保的工艺，已被广泛研究以提高木质纤维素生物质的可消化性。然而，在真菌预处理之前，通常需要对原料进行消毒，这是一个昂贵的过程。在本研究中，使用预先被 Ceriporiopsis subvermispora 定植的庭院修剪物作为接种物对未经消毒的庭院修剪物进行了真菌预处理。在真菌预处理的 30 天内，使用不同的接种物/底物比（1:19、1:9 和 1:4），庭院修剪物中的木质素、纤维素、半纤维素和干物质的降解率分别为 14.8-20.2%、8.1-15.4%、20.7-27.8%和 9.8-16.2%。预处理后的庭院修剪物在固态厌氧消化（SS-AD）中的甲烷产率为 34.9-44.6L/kg 挥发性固体，与传统方法（需要对原料进行消毒）相比，这一结果相当。本研究中开发的技术可以节省约 501-789 kJ/kg 干燥的庭院修剪物处理过程中的能量，这大约是 SS-AD 产生的总沼气能量的一半。
