Department of Food, Agricultural and Biological Engineering, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691, USA; Environmental Science Graduate Program, The Ohio State University, 3138A Smith Lab, 174 West 18th, Columbus, OH 43210, USA.
Department of Food, Agricultural and Biological Engineering, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691, USA; Quasar Energy Group, 8600 E. Pleasant Valley Rd, Independence, OH 44131, USA.
Bioresour Technol. 2017 Jul;236:186-193. doi: 10.1016/j.biortech.2017.03.136. Epub 2017 Mar 27.
Sequential batch thermophilic (55°C) solid-state anaerobic digestion (SS-AD) of yard trimmings was evaluated at a total solids (TS) content of 22% via recirculating digestate as the inoculum. The substrate-to-inoculum (S/I) ratio of 1 (TS basis) was favored over 2 and 3 due to significantly higher methane yield and volumetric productivity. At an S/I ratio of 1, sequential batch SS-AD gradually reached steady state by 3 runs (30days/run) with increases in both methane yields (up to 11.5%) and cellulose degradation (up to 55%), indicating that recirculated digestate could be a feasible inoculum to establish long term stable SS-AD of lignocellulosic biomass. The initial sharp increases of volatile fatty acids during runs 2-4 indicated faster hydrolysis of organic matter than during run 1, suggesting that microbes were probably more acclimated due to digestate recirculation. At steady state, 51% (w/w) of the digestate was recirculated as the inoculum.
采用 22%总固体（TS）含量的循环消化液作为接种物，评估了庭院修剪物在序批式高温（55°C）固态厌氧消化（SS-AD）中的性能。由于甲烷产量和容积产率显著提高，底物与接种物（S/I）比为 1（TS 基础）优于 2 和 3。在 S/I 比为 1 的情况下，通过 3 次运行（每次运行 30 天），序批式 SS-AD 逐渐达到稳定状态，甲烷产量（高达 11.5%）和纤维素降解（高达 55%）均有所增加，表明循环消化液可用作建立木质纤维素生物质长期稳定 SS-AD 的可行接种物。在运行 2-4 期间，挥发性脂肪酸的初始急剧增加表明有机物质的水解速度比运行 1 更快，这表明由于消化液的循环，微生物可能更适应。在稳定状态下，51%（w/w）的消化液作为接种物循环使用。
