LINA 队列研究：脐血嗜酸性粒细胞/嗜碱性粒细胞祖细胞可预测婴儿早期的呼吸结局。
The LINA cohort: Cord blood eosinophil/basophil progenitors predict respiratory outcomes in early infancy.
机构信息
Helmholtz Centre for Environmental Research - UFZ, Leipzig, Department of Environmental Immunology, Germany; McMaster University, Division of Clinical Immunology and Allergy, Hamilton, ON, Canada.
Helmholtz Centre for Environmental Research - UFZ, Leipzig, Department of Environmental Immunology, Germany.
出版信息
Clin Immunol. 2014 May-Jun;152(1-2):68-76. doi: 10.1016/j.clim.2014.02.013. Epub 2014 Mar 4.
RATIONALE
Cord blood eosinophil/basophil progenitor cells (Eo/B) of high risk infants have been shown to predict respiratory illnesses in infancy. Here we investigated this association in a population-based cohort. Furthermore, we analysed whether newborns Th1/Th2 balance and prenatal environmental exposure impact Eo/B recruitment.
METHODS
In a sub-cohort of the LINA study cord blood mononuclear cells were used for methylcellulose assays to assess Eo/B differentiation. Questionnaires were recorded during pregnancy and annually thereafter. Volatile organic compounds were measured during pregnancy and cord blood cytokines after ex vivo stimulation.
RESULTS
Cord blood IL-4 and IL-13 positively correlated with Eo/B. Tobacco smoke related benzene was also positively associated with Eo/B. Enhanced Eo/B numbers increased the risk for wheezing within the first 24 months.
CONCLUSIONS
The association between cord blood Eo/B and respiratory illnesses is not restricted to high-risk children. Prenatal environmental exposure and a Th2 milieu at birth contribute to Eo/B recruitment.
背景
高危婴儿的脐带血嗜酸性粒细胞/嗜碱性粒细胞祖细胞（Eo/B）已被证明可预测婴儿期的呼吸道疾病。在此，我们在基于人群的队列中研究了这种关联。此外，我们还分析了新生儿 Th1/Th2 平衡和产前环境暴露是否会影响 Eo/B 的募集。
方法
在 LINA 研究的子队列中，使用脐带血单核细胞进行甲基纤维素测定以评估 Eo/B 的分化。在怀孕期间和之后每年都会记录问卷调查。在怀孕期间测量挥发性有机化合物，在体外刺激后测量脐带血细胞因子。
结果
脐带血中的 IL-4 和 IL-13 与 Eo/B 呈正相关。与吸烟有关的苯也与 Eo/B 呈正相关。增强的 Eo/B 数量增加了前 24 个月喘息的风险。
结论
脐带血 Eo/B 与呼吸道疾病的关联不仅限于高危儿童。产前环境暴露和出生时的 Th2 环境有助于 Eo/B 的募集。
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