Altered exocrine pancreatic function in rats treated with nicotine.

The present study investigates the effects of nicotine treatment on exocrine pancreatic function. Adult male, Sprague-Dawley rats received nicotine via a time-release pellet, at a rate of 1.65 micrograms/min for 3 weeks. At the end of the experimental period, it was observed that although nicotine did not affect final body or pancreatic weight, the activities of amylase, trypsin, and chymotrypsin in pancreatic homogenates from nicotine-treated rats were 51, 29, and 35% higher, respectively, than in controls. Levels of immunoreactive cationic trypsin(ogen) were significantly higher in pancreatic homogenates and serum from nicotine-treated rats as compared with controls. In addition, concentrations of mRNA, encoding for pancreatic amylase, were higher in pancreatic homogenates from the nicotine-treated rats than in controls. In dispersed pancreatic acini isolated from nicotine-treated rats, basal secretion of amylase, trypsinogen, and chymotrypsinogen was 50% higher than controls and enzyme release following CCK-8 (100 pM), secretin (1 microM), and carbachol (7.5 microM) stimulation was also significantly higher. These data indicate that nicotine treatment, at levels comparable to those expected in moderate cigarette smokers, increases the content of digestive enzymes in rat pancreas, as well as their basal and secretagogue-induced release.