Wang F, Chang G-M, Geng X
Department of Neurology, General Hospital, Tianjin Medical University, China.
Eur Rev Med Pharmacol Sci. 2014;18(4):526-36.
Alternative splicing of human telomerase reverse transcriptase (hTERT) has an important effect on regulating telomerase activity. Exonic splicing enhancers (ESEs) are a family of conserved splicing factors that participate in multiple steps of the splicing pathway. Our aim is to analyze the ESEs for predicting the potential regulatory elements of hTERT mRNA splicing.
Enter the FASTA format of hTERT total sequences or individual exon as the input data in the main interface of ESEfinder3.0 and ESEfinder2.0 program. Analyze the data of output results and compare the differences between ESEfinder3.0 and ESEfinder2.0 program.
Five ESEs were predicted in exon 5 to exon 9 of hTERT. They were at position 108 located in hTERT exon 5, at position 92 located in exon 6, at position 22 located in exon 7, at position 73 located in exon 8 and at position 5 located in exon 9. There were no differences between ESEfinder 3.0 and ESEfinder 2.0 in our case.
The identification of these potential ESEs of hTERT might be helpful for the design of antisense oligonucleotides, which could modulate hTERT alternative splicing and inhibit telomerase activity.
人端粒酶逆转录酶（hTERT）的可变剪接对调节端粒酶活性具有重要作用。外显子剪接增强子（ESEs）是一类保守的剪接因子，参与剪接途径的多个步骤。我们的目的是分析ESEs，以预测hTERT mRNA剪接的潜在调控元件。
将hTERT总序列或单个外显子的FASTA格式作为输入数据，输入到ESEfinder3.0和ESEfinder2.0程序的主界面中。分析输出结果的数据，并比较ESEfinder3.0和ESEfinder2.0程序之间的差异。
在hTERT的外显子5至外显子9中预测到5个ESEs。它们分别位于hTERT外显子5的第108位、外显子6的第92位、外显子7的第22位、外显子8的第73位和外显子9的第5位。在我们的案例中，ESEfinder 3.0和ESEfinder 2.0之间没有差异。
鉴定这些hTERT潜在的ESEs可能有助于反义寡核苷酸的设计，其可调节hTERT的可变剪接并抑制端粒酶活性。
