Center for Cancer Research (CIC-IBMCC; CSIC/USAL), IBSAL, Cytometry service (NUCLEUS), Department of Medicine, University of Salamanca, Salamanca, Spain; Center for Neurosciences and Cell Biology and Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
Neuropathol Appl Neurobiol. 2015 Apr;41(3):319-32. doi: 10.1111/nan.12127.
Limited information exists about the impact of cytogenetic alterations on the protein expression profiles of individual meningioma cells and their association with the clinicohistopathological characteristics of the disease. The aim of this study is to investigate the potential association between the immunophenotypic profile of single meningioma cells and the most relevant features of the tumour.
Multiparameter flow cytometry (MFC) was used to evaluate the immunophenotypic profile of tumour cells (n = 51 patients) and the Affymetrix U133A chip was applied for the analysis of the gene expression profile (n = 40) of meningioma samples, cytogenetically characterized by interphase fluorescence in situ hybridization.
Overall, a close association between the pattern of protein expression and the cytogenetic profile of tumour cells was found. Thus, diploid tumours displayed higher levels of expression of the CD55 complement regulatory protein, tumours carrying isolated monosomy 22/del(22q) showed greater levels of bcl2 and PDGFRβ and meningiomas carrying complex karyotypes displayed a greater proliferation index and decreased expression of the CD13 ectoenzyme, the CD9 and CD81 tetraspanins, and the Her2/neu growth factor receptor. From the clinical point of view, higher expression of CD53 and CD44 was associated with a poorer outcome.
Here we show that the protein expression profile of individual meningioma cells is closely associated with tumour cytogenetics, which may reflect the involvement of different signalling pathways in the distinct cytogenetic subgroups of meningiomas, with specific immunophenotypic profiles also translating into a different tumour clinical behaviour.
关于细胞遗传学改变对个别脑膜瘤细胞的蛋白质表达谱的影响及其与该疾病的临床病理特征的关系，相关信息有限。本研究旨在探讨单个脑膜瘤细胞的免疫表型特征与肿瘤最相关特征之间的潜在关联。
采用多参数流式细胞术（MFC）评估肿瘤细胞的免疫表型特征（n=51 例患者），并应用 Affymetrix U133A 芯片分析脑膜瘤样本的基因表达谱（n=40 例），这些样本通过间期荧光原位杂交进行细胞遗传学特征分析。
总体而言，发现蛋白质表达模式与肿瘤细胞的细胞遗传学特征之间存在密切关联。因此，二倍体肿瘤显示出更高水平的 CD55 补体调节蛋白表达，携带孤立单体 22/缺失 22q 的肿瘤显示出更高水平的 bcl2 和 PDGFRβ，而具有复杂核型的脑膜瘤则显示出更高的增殖指数和更低的 CD13 细胞外酶、CD9 和 CD81 四跨膜蛋白以及 Her2/neu 生长因子受体表达。从临床角度来看，CD53 和 CD44 的高表达与预后不良相关。
在这里，我们表明单个脑膜瘤细胞的蛋白质表达谱与肿瘤细胞遗传学密切相关，这可能反映了不同信号通路在脑膜瘤不同细胞遗传学亚群中的参与，特定的免疫表型特征也转化为不同的肿瘤临床行为。
