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一种可能参与小鼠腹水肉瘤细胞中博来霉素损伤DNA修复起始过程的核酸外切酶。

An exonuclease possibly involved in the initiation of repair of bleomycin-damaged DNA in mouse ascites sarcoma cells.

作者信息

Seki S, Oda T

机构信息

Department of Biochemistry, Okayama University Medical School, Japan.

出版信息

Carcinogenesis. 1988 Dec;9(12):2239-44. doi: 10.1093/carcin/9.12.2239.

DOI:10.1093/carcin/9.12.2239
PMID:2461264
Abstract

A protein factor having exonucleolytic activity on bleomycin-damaged DNA and providing priming sites for DNA polymerases existed in a DNA polymerase beta fraction partially purified by ion exchange chromatography from an extract of permeable mouse ascites sarcoma (SR-C3H/He) cells. The exonuclease was separated from DNA polymerase beta by single-stranded DNA-cellulose chromatography, and partially characterized. The enzyme is suggested to be involved in the initial step of repair of bleomycin-damaged DNA in removing 3' ends (3'-phosphoglycolate termini) of bleomycin-damaged DNA.

摘要

一种对博来霉素损伤的DNA具有核酸外切酶活性并为DNA聚合酶提供引物位点的蛋白质因子存在于通过离子交换色谱法从可渗透的小鼠腹水肉瘤（SR-C3H/He）细胞提取物中部分纯化得到的DNA聚合酶β组分中。通过单链DNA-纤维素色谱法将该核酸外切酶与DNA聚合酶β分离，并对其进行了部分特性鉴定。该酶被认为参与了博来霉素损伤DNA修复的初始步骤，即去除博来霉素损伤DNA的3'末端（3'-磷酸乙醇酸末端）。

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