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Repair of DNA damaged by UV light and ionizing radiation by cell-free extracts prepared from Schizosaccharomyces pombe.

作者信息

Sidik K, Lieberman H B, Freyer G A

机构信息

Center for Radiological Research, Columbia University, New York, NY 10032.

出版信息

Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):12112-6. doi: 10.1073/pnas.89.24.12112.

DOI:10.1073/pnas.89.24.12112
PMID:1465447
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC50708/
Abstract

A whole cell extract prepared from Schizosaccharomyces pombe was shown to be active in an assay for repair of plasmid DNA damaged by either ultraviolet (UV) light or gamma-radiation. The assay allows for analysis of repair synthesis at single-strand nicks generated by gamma-rays and analysis of the incision step and repair synthesis in UV-light-damaged DNA. Repair synthesis of DNA damaged by either UV light or gamma-rays was shown to depend on the presence of ATP in the reaction mixture. However, incision at pyrimidine dimers did not require the addition of exogenous ATP. These studies showed that plasmid DNA containing a single pyrimidine dimer or one single-strand nick is a suitable substrate in this assay system. S. pombe is a genetically well-defined eukaryotic organism and many radiation-sensitive mutant derivatives have already been described, making this a powerful system in which to study DNA excision repair.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/362432138a2c/pnas01098-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/842f47c75612/pnas01098-0474-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/12bd260af6fc/pnas01098-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/48bdf5c7ee9e/pnas01098-0475-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/24dfd649d559/pnas01098-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/362432138a2c/pnas01098-0476-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/842f47c75612/pnas01098-0474-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/12bd260af6fc/pnas01098-0475-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/48bdf5c7ee9e/pnas01098-0475-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/24dfd649d559/pnas01098-0476-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ef5/50708/362432138a2c/pnas01098-0476-b.jpg

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本文引用的文献

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gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.γ射线诱导的脱氧核糖核酸链断裂。3' 乙醇酸末端。
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A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region.一种新型修复酶：大肠杆菌的UVRABC切除核酸酶在受损区域两侧切割DNA链。
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Saccharomyces cerevisiae exonuclease-1 plays a role in UV resistance that is distinct from nucleotide excision repair.酿酒酵母核酸外切酶-1在紫外线抗性中发挥作用，这一作用与核苷酸切除修复不同。
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Role of Schizosaccharomyces pombe RecQ homolog, recombination, and checkpoint genes in UV damage tolerance.粟酒裂殖酵母RecQ同源物、重组及检查点基因在紫外线损伤耐受中的作用。
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The rad18 gene of Schizosaccharomyces pombe defines a new subgroup of the SMC superfamily involved in DNA repair.粟酒裂殖酵母的rad18基因定义了参与DNA修复的SMC超家族的一个新亚组。
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A new ATP-independent DNA endonuclease from Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers and 6-4 photoproducts.一种来自粟酒裂殖酵母的新型不依赖ATP的DNA内切核酸酶，它能识别环丁烷嘧啶二聚体和6-4光产物。
Nucleic Acids Res. 1994 Aug 11;22(15):3026-32. doi: 10.1093/nar/22.15.3026.
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An alternative eukaryotic DNA excision repair pathway.一种替代性真核生物DNA切除修复途径。
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J Biol Chem. 1985 Nov 15;260(26):14214-23.
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Recovery, repair, and mutagenesis in Schizosaccharomyces pombe.粟酒裂殖酵母中的恢复、修复和诱变
Adv Genet. 1985;23:1-72. doi: 10.1016/s0065-2660(08)60511-8.
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Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts.无细胞提取物中着色性干皮病DNA修复缺陷的互补作用。
Cell. 1988 Apr 8;53(1):97-106. doi: 10.1016/0092-8674(88)90491-6.
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DNA repair enzymes.DNA修复酶
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The purification of the Escherichia coli UvrABC incision system.大肠杆菌UvrABC切割系统的纯化
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DNA topoisomerases.DNA拓扑异构酶
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