Habouzit Frédéric, Hamelin Jérôme, Santa-Catalina Gaëlle, Steyer Jean-P, Bernet Nicolas
Laboratoire de Biotechnologie de l'Environnement, Institut National de la Recherche Agronomique, Narbonne, 11100, France.
Microb Biotechnol. 2014 May;7(3):257-64. doi: 10.1111/1751-7915.12115. Epub 2014 Feb 25.
To evaluate the impact of the nature of the support material on its colonization by a methanogenic consortium, four substrata made of different materials: polyvinyl chloride, 2 polyethylene and polypropylene were tested during the start-up of lab-scale fixed-film reactors. The reactor performances were evaluated and compared together with the analysis of the biofilms. Biofilm growth was quantified and the structure of bacterial and archaeal communities were characterized by molecular fingerprinting profiles (capillary electrophoresis-single strand conformation polymorphism). The composition of the inoculum was shown to have a major impact on the bacterial composition of the biofilm, whatever the nature of the support material or the organic loading rate applied to the reactors during the start-up period. In contrast, the biofilm archaeal populations were independent of the inoculum used but highly dependent on the support material. Supports favouring Archaea colonization, the limiting factor in the overall process, should be preferred.
为评估载体材料性质对产甲烷菌群落定殖的影响，在实验室规模的固定膜反应器启动阶段，测试了由不同材料制成的四种载体：聚氯乙烯、两种聚乙烯和聚丙烯。对反应器性能进行了评估，并与生物膜分析结果进行了比较。对生物膜生长进行了定量分析，并通过分子指纹图谱（毛细管电泳-单链构象多态性）对细菌和古菌群落结构进行了表征。结果表明，无论载体材料的性质如何，也无论在启动阶段施加于反应器的有机负荷率如何，接种物的组成对生物膜的细菌组成都有重大影响。相比之下，生物膜古菌种群与所用接种物无关，但高度依赖于载体材料。应优先选择有利于古菌定殖的载体，因为古菌定殖是整个过程中的限制因素。
