Department of Biological Sciences, School of Dental Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
Biol Proced Online. 2014 Mar 10;16(1):4. doi: 10.1186/1480-9222-16-4.
Utilizing mouse models provides excellent immunological and experimental tools to study oral immune responses. However for functional assays, isolating T lymphocytes from the oral tissues has proved to be challenging due to the absence of reliable methods that yield viable cells with consistency. To study adaptive immune cell interactions in the oral mucosal tissues, it is necessary to isolate T cells with a good viability and study them at the single cell level.
We have established an improved method to isolate immune cells, including Tregs and Th17 cells from intra-epithelial niches and lamina propria of the tongue, gingival and palatal tissues in the oral mucosa of mice.
This new method of isolating immune cells from oral tissues will enable us to further our understanding of oral tissue immune cells and their role during oral infections and oral inflammation.
利用小鼠模型为研究口腔免疫反应提供了出色的免疫学和实验工具。然而，对于功能分析而言，由于缺乏可靠的方法来一致地获得具有活力的细胞，因此从口腔组织中分离 T 淋巴细胞一直具有挑战性。为了研究口腔黏膜组织中的适应性免疫细胞相互作用，有必要分离具有良好活力的 T 细胞，并在单细胞水平上对其进行研究。
我们已经建立了一种改良的方法，可从口腔黏膜的舌上皮内巢、固有层、牙龈和腭组织中分离免疫细胞，包括 Tregs 和 Th17 细胞。
这种从口腔组织中分离免疫细胞的新方法将使我们能够进一步了解口腔组织免疫细胞及其在口腔感染和口腔炎症期间的作用。
