Hay D L, Lopata A
Department of Pathology, Royal Women's Hospital, Melbourne, Australia.
J Clin Endocrinol Metab. 1988 Dec;67(6):1322-4. doi: 10.1210/jcem-67-6-1322.
Embryos (n = 38) from an in vitro fertilization (IVF) program, unsuitable for transfer or cryopreservation, were maintained in culture for 14 days. The time course of hCG secretion into culture fluid was compared to observed embryo development. The earliest significant hCG rise was detected at day 7 post insemination for a partially hatched blastocyst, with hCG levels increasing 50-fold following blastocyst attachment to the surface of the culture vessel. Secretion of hCG was amplified by the addition of serum. There was no significant free alpha hCG or beta hCG secretion between days 6-12. We conclude that hCG production depends on the pathway of cytotrophoblast differentiation and commences with the appearance of the G1 phase in embryonic cells at the late blastocyst stage.
来自体外受精（IVF）项目的38个胚胎，因不适宜移植或冷冻保存，在培养中维持14天。将培养液中hCG分泌的时间进程与观察到的胚胎发育进行比较。对于一个部分孵化的囊胚，在授精后第7天检测到最早的显著hCG升高，囊胚附着于培养容器表面后hCG水平增加50倍。添加血清可增强hCG的分泌。在第6 - 12天之间没有显著的游离α-hCG或β-hCG分泌。我们得出结论，hCG的产生取决于细胞滋养层分化途径，并始于囊胚晚期胚胎细胞中G1期的出现。
