多余人类胚胎：其在培养中的生长、囊胚形成、孵化及人绒毛膜促性腺激素产生的潜力。

The surplus human embryo: its potential for growth, blastulation, hatching, and human chorionic gonadotropin production in culture.

作者信息

Lopata A, Hay D L

机构信息

Department of Obstetrics and Gynecology, University of Melbourne, Victoria, Australia.

出版信息

Fertil Steril. 1989 Jun;51(6):984-91. doi: 10.1016/s0015-0282(16)60731-8.

DOI:10.1016/s0015-0282(16)60731-8

PMID:2721733

Abstract

Early embryos that were unsuitable for transfer to patients or for cryopreservation were cultured either in a human tubal fluid (HTF) or a minimum essential medium (MEM). A significantly higher proportion of embryos developed to blastocysts in MEM (26.8%) than in HTF (14.5%). Approximately similar proportions of embryos formed blastocysts in MEM in the presence or absence of serum. The rate of embryo growth to blastocysts was similar in all media. Blastocyst hatching occurred in MEM + or - serum, but it failed to occur in HTF with serum. Released human chorionic gonadotropin (hCG) from hatched and intrazonal blastocysts was detected by day 8 after fertilization. The mean amount of hCG produced by day 14 was 19,500 mIU from hatched and 1,550 mIU from intrazonal blastocysts. Serum stimulated the output of hCG.

摘要

不适合移植给患者或冷冻保存的早期胚胎，要么在人输卵管液（HTF）中培养，要么在最低必需培养基（MEM）中培养。在MEM中发育成囊胚的胚胎比例（26.8%）显著高于在HTF中（14.5%）。在有血清或无血清的情况下，MEM中形成囊胚的胚胎比例大致相似。在所有培养基中，胚胎发育到囊胚的速率相似。囊胚孵化在含或不含血清的MEM中均可发生，但在含血清的HTF中未发生。在受精后第8天可检测到从孵化的和透明带内囊胚释放的人绒毛膜促性腺激素（hCG）。到第14天，孵化囊胚产生的hCG平均量为19,500 mIU，透明带内囊胚产生的为1,550 mIU。血清刺激hCG的分泌。