Harris J E, Kretchmer N
Department of Nutritional Sciences, University of California, Berkeley 94720.
J Nutr. 1988 Nov;118(11):1319-24. doi: 10.1093/jn/118.11.1319.
The accumulation of hepatic protein during pregnancy in the rat could be the result of a decrease in rate of degradation, an increase in rate of synthesis or both. The purpose of the study was to determine relative rates of synthesis of hepatic protein for pregnant and nonpregnant rats. Pregnant and nonpregnant Sprague-Dawley rats were fed a 20% casein, purified diet from the first day of gestation. On d 19 of gestation the rats were killed. Livers were weighed and assayed for RNA. Relative synthesis rates of hepatic protein were assessed by homologous in vitro cell-free translation. RNA per liver and per g liver and incorporation of labeled leucine into protein per liver and per unit of RNA were greater for the pregnant rats than the nonpregnant rats. In conclusion, accumulation of hepatic protein during pregnancy is at least partially the result of an increase in rate of synthesis.
大鼠孕期肝脏蛋白质的积累可能是降解速率降低、合成速率增加或两者共同作用的结果。本研究的目的是确定怀孕大鼠和未怀孕大鼠肝脏蛋白质的相对合成速率。从妊娠第一天起，给怀孕和未怀孕的斯普拉格-道利大鼠喂食含20%酪蛋白的纯化日粮。在妊娠第19天处死大鼠。称量肝脏重量并检测RNA。通过同源体外无细胞翻译评估肝脏蛋白质的相对合成速率。怀孕大鼠每肝脏和每克肝脏的RNA以及每肝脏和每单位RNA中标记亮氨酸掺入蛋白质的量均高于未怀孕大鼠。总之，孕期肝脏蛋白质的积累至少部分是合成速率增加的结果。
