Genos Glycoscience Laboratory, Zagreb, Croatia.
Department of Orthopaedic Surgery University Hospital Centre Zagreb, Croatia.
Sci Rep. 2014 Mar 11;4:4347. doi: 10.1038/srep04347.
Recovery after cardiac surgery is a complex process that has to compensate for both individual variability and extensive tissue damage in the context of systemic inflammation. Protein glycosylation is essential in many steps of the inflammatory cascade, but due to technological limitations the role of individual variation in glycosylation in systemic inflammation has not been addressed until now. We analysed composition of the total plasma and IgG N-glycomes in 107 patients undergoing cardiac surgery. In nearly all individuals plasma N-glycome underwent the same pattern of changes in the first 72 h, revealing a general mechanism of glycosylation changes. To the contrary, changes in the IgG glycome were very individualized. Bi-clustering analysis revealed the existence of four distinct patterns of changes. One of them, characterized by a rapid increase in galactosylated glycoforms, was associated with nearly double mortality risk measured by EuroSCORE II. Our results indicate that individual variation in IgG glycosylation changes during acute systemic inflammation associates with increased mortality risk and indicates new avenues for the development of personalized diagnostic and therapeutic approach.
心脏手术后的恢复是一个复杂的过程，必须在全身炎症的背景下补偿个体差异和广泛的组织损伤。蛋白糖基化在炎症级联的许多步骤中都是必不可少的，但由于技术限制，个体糖基化变异在全身炎症中的作用直到现在才得到解决。我们分析了 107 例接受心脏手术的患者的总血浆和 IgG N-糖组。在几乎所有个体中，血浆 N-糖组在最初的 72 小时内经历了相同的变化模式，揭示了一种普遍的糖基化变化机制。相反，IgG 糖组的变化非常个体化。双聚类分析显示存在四种不同的变化模式。其中一种模式的特点是半乳糖基化糖型迅速增加，与通过 EuroSCORE II 测量的死亡率风险几乎增加一倍有关。我们的结果表明，急性全身炎症期间 IgG 糖基化变化的个体差异与死亡率风险增加相关，并为开发个性化诊断和治疗方法指明了新途径。
