Department of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia.
Genos Glycoscience Research Laboratory, 10000 Zagreb, Croatia.
Biomolecules. 2023 Feb 16;13(2):375. doi: 10.3390/biom13020375.
Coronary artery disease (CAD) is the most common cardiovascular disease (CVD), and previous studies have shown a significant association between N-glycosylation, a highly regulated posttranslational modification, and the development of atherosclerotic plaques. Our aim was to determine whether the N-glycome of immunoglobulin G (IgG) is associated with CAD, as N-glycans are known to alter the effector functions of IgG, which may enhance the inflammatory response in CAD. Therefore, in this study, we isolated IgG from subjects with coronary atherosclerosis (CAD+) and from subjects with clean coronaries (CAD-). The purified IgGs were denatured and enzymatically deglycosylated, and the released and fluorescently labelled N-glycans were analysed by ultra-high performance liquid chromatography based on hydrophilic interactions with fluorescence detection (HILIC-UHPLC-FLR). Sex-stratified analysis of 316 CAD- and 156 CAD+ cases revealed differences in IgG N-glycome composition. The most notable differences were observed in women, where the presence of sialylated N-glycan structures was negatively associated with CAD. The obtained chromatograms provide insight into the IgG N-glycome composition in CAD as well as the biomarker potential of IgG N-glycans in CAD.
冠状动脉疾病（CAD）是最常见的心血管疾病（CVD），先前的研究表明，高度调控的翻译后修饰 N-糖基化与动脉粥样硬化斑块的发展之间存在显著关联。我们的目的是确定 IgG 的 N-聚糖是否与 CAD 相关，因为众所周知，N-聚糖可改变 IgG 的效应功能，从而增强 CAD 中的炎症反应。因此，在这项研究中，我们从冠状动脉粥样硬化（CAD+）患者和冠状动脉无病变（CAD-）患者中分离出 IgG。将纯化的 IgG 变性并酶促去糖基化，然后通过基于亲水相互作用与荧光检测的超高效液相色谱法（HILIC-UHPLC-FLR）分析释放和荧光标记的 N-聚糖。对 316 名 CAD-和 156 名 CAD+病例进行的性别分层分析显示，IgG N-聚糖组成存在差异。在女性中观察到最显著的差异，其中唾液酸化的 N-聚糖结构的存在与 CAD 呈负相关。所得色谱图提供了对 CAD 中 IgG N-聚糖组成的深入了解，以及 IgG N-聚糖在 CAD 中的生物标志物潜力。
