Pliusnin A Z, Nolandt O V, Kuznetsova E V, Briantseva E A, Fridman E A
Zh Mikrobiol Epidemiol Immunobiol. 1988 Aug(8):19-23.
DNA probes containing the nucleotide sequences of the conservative genes of influenza A virus (matrix, nucleoprotein and acidic polymerase genes) show their specificity with respect to the RNA of influenza A viruses in mammal tissue cell cultures (continuous spaniel kidney cell culture and primary calf kidney cell culture). The minimal amount of infected monolayer cells, permitting the detection of viral RNA, is 10(3). The results obtained in the study of nasopharyngeal washings make it possible to recommend the method of molecular hybridization for use in the epidemiological analysis in addition to virological and serological tests. The method of hybridization permits the detection of virus-specific RNA in the allantoic fluid of chick embryos in subculturing the materials under study even in those cases when hemagglutinating influenza virus cannot be isolated.
含有甲型流感病毒保守基因（基质、核蛋白和酸性聚合酶基因）核苷酸序列的DNA探针，在哺乳动物组织细胞培养物（连续的西班牙猎犬肾细胞培养物和原代小牛肾细胞培养物）中，对甲型流感病毒的RNA显示出特异性。能够检测到病毒RNA的感染单层细胞的最小量为10³。在对鼻咽冲洗液的研究中获得的结果表明，除病毒学和血清学检测外，分子杂交方法可推荐用于流行病学分析。杂交方法能够在对研究材料进行传代培养时，检测鸡胚尿囊液中病毒特异性RNA，即使在无法分离出血凝性流感病毒的情况下也是如此。
