Pljusnin A Z, Rozhkova S A, Nolandt O V, Bryantseva E A, Kuznetsov O K, Noskov F S
Virologie. 1987 Apr-Jun;38(2):111-4.
The possibilities of using DNA-copies of different influenza A virus genes cloned with recombinant bacterial plasmids for the detection of virus-specific RNA by molecular dot-hybridization were analyzed. High specificity of RNA identification has been demonstrated and it has been shown expedient to use DNA-probes with high-conservative virus genes (polymerase, nucleoprotein, or matrix) for the detection of influenza A virus subtypes (H1N1, H2N2, H3N2) and probes with corresponding hemagglutinin genes for the differentiation of the subtypes H3N2 and H1N1. The results of nasopharyngeal specimens testing proved the effectiveness of molecular dot-hybridization in epidemiological studies of influenza outbreaks, especially of mixed etiology.
分析了利用重组细菌质粒克隆的不同甲型流感病毒基因的DNA拷贝通过分子点杂交检测病毒特异性RNA的可能性。已证明RNA鉴定具有高度特异性，并且已表明使用具有高保守病毒基因（聚合酶、核蛋白或基质）的DNA探针来检测甲型流感病毒亚型（H1N1、H2N2、H3N2）以及使用具有相应血凝素基因的探针来区分H3N2和H1N1亚型是适宜的。鼻咽标本检测结果证明了分子点杂交在流感暴发流行病学研究，尤其是混合病因研究中的有效性。
