Hawash Yousry
Department of Medical Parasitology, National Liver Institute, Menoufia University, Shebin-El Koum 32513, Menoufia, Egypt ; Department of Medical Laboratory Science, College of Applied Medical Science, Taif University, Taif 2425, Saudi Arabia.
J Parasitol Res. 2014;2014:213745. doi: 10.1155/2014/213745. Epub 2014 Jan 29.
An immunoassay-based algorithm, involving three commercial kits, was introduced and evaluated for screening and identification of Giardia/Cryptosporidium antigens in human stool specimens. Initially, Giardia/Cryptosporidium Chek kit (TechLab), an enzyme-linked immunosorbent assay (ELISA), was adopted for screening. The ELISA-positive reactions were subsequently characterised by RIDA Quick Giardia and RIDA Quick Cryptosporidium immunochromatographic kits (R-Biopharm). A gold standard test comprising PCR and microscopy was used for preparing control samples. Performance of individual kits was tested against these samples which included 50 Giardia-positive, 40 Cryptosporidium-positive, and 70 Cryptosporidium/Giardia-negative. For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively. Both kits demonstrated sensitivity of 95%. For Giardia, the ELISA and RIDA Quick Giardia kits showed sensitivities of 100% and 97.5%, respectively. Specificities obtained by the ELISA and RIDA Quick Giardia were 95.7% and 100%, respectively. Based on the results of two reference PCRs, on 250 random samples, the algorithm exhibited sensitivity, specificity, positive predictive value, and negative predictive value of 97.06%, 100.00%, 100.00%, and 98.91%, respectively. In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.
一种基于免疫测定的算法被引入并评估，该算法涉及三种商业试剂盒，用于筛查和鉴定人类粪便标本中的贾第虫/隐孢子虫抗原。最初，采用贾第虫/隐孢子虫检测试剂盒（TechLab），一种酶联免疫吸附测定（ELISA）进行筛查。随后，通过RIDA Quick贾第虫和RIDA Quick隐孢子虫免疫层析试剂盒（R-Biopharm）对ELISA阳性反应进行特征分析。采用包括PCR和显微镜检查的金标准试验来制备对照样本。针对这些包括50份贾第虫阳性、40份隐孢子虫阳性和70份隐孢子虫/贾第虫阴性的样本，测试了各个试剂盒的性能。对于隐孢子虫，ELISA试剂盒和RIDA Quick隐孢子虫试剂盒的特异性分别为95.71%和100%。两种试剂盒的灵敏度均为95%。对于贾第虫，ELISA试剂盒和RIDA Quick贾第虫试剂盒的灵敏度分别为100%和97.5%。ELISA试剂盒和RIDA Quick贾第虫试剂盒的特异性分别为95.7%和100%。基于两次参考PCR的结果，在250份随机样本上，该算法的灵敏度、特异性、阳性预测值和阴性预测值分别为97.06%、100.00%、100.00%和98.91%。总之，这种基于免疫测定的算法可作为诊断实验室中用于大量样本筛查和鉴定的常规检测方法。
