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通过对致倦库蚊进行显性致死试验评估乙酰甲胺磷和毒死蜱的潜在毒性。

Evaluation of toxic potential of acephate and chlorpyrifos by dominant lethal test on Culex quinquefasciatus.

作者信息

Bhinder Preety, Chaudhry Asha

出版信息

J Environ Biol. 2013 May;34(3):573-7.

Abstract

The present paper deals with the toxicity evaluation of pesticides acephate and chlorpyrifos by applying dominant lethal test (DLT) on mosquito Culex quinquefasciatus taken as an experimental model. For this, the adult male mosquitoes emerging from LC20 treated larval stock were allowed to crossmate with normal virgin females under controlled conditions of mosquito rearing laboratory along with the parallel controls, separately for each pesticide. The eggs obtained from such females were allowed to hatch after which they were examined under suitable magnification. The number of unhatched eggs was taken as the measure for calculating the dominant lethality caused by the pesticides and the data was analyzed statistically by applying Student's t-test. The statistical analysis of the results for acephate treated groups was 9.49 +/- 1.50 as against 3.92 +/- 0.41 in the control groups and chlorpyrifos treated groups gave the value 9.94 +/- 1.92 as against 4.26 +/- 0.35 in the control groups. The results indicated that these pesticides induced significant (p < 0.05) dominant lethality.

摘要

本文以致倦库蚊为实验模型,通过显性致死试验(DLT)对农药乙酰甲胺磷和毒死蜱进行毒性评估。为此,将从经LC20处理的幼虫种群中羽化出的成年雄蚊,在蚊虫饲养实验室的受控条件下,分别与正常未交配的雌蚊进行杂交,并设置平行对照组,每种农药单独进行实验。从这些雌蚊获得的卵孵化后,在适当放大倍数下进行检查。未孵化卵的数量作为计算农药引起的显性致死率的指标,并应用学生t检验对数据进行统计分析。乙酰甲胺磷处理组结果的统计分析为9.49±1.50,而对照组为3.92±0.41;毒死蜱处理组的值为9.94±1.92,对照组为4.26±0.35。结果表明,这些农药诱导了显著的(p<0.05)显性致死率。

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