Shiokawa K, Tashiro K, Nakakura N, Fu Y, Atsuchi Y, Nakazato S, Tsuzaki Y, Ikenishi K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Cell Differ Dev. 1988 Sep;25(1):47-55. doi: 10.1016/0922-3371(88)90054-8.
Embryos from a female of Xenopus laevis (designated as no. 65) arrest development at gastrulation and are assumed to be ova-deficient mutant. We dissociated these embryos and studied RNA synthesis at different stages. The cells from the ova-deficient embryos reaggregated quite actively as wild-type embryo cells until the late gastrula stage. RNA synthesis was normal at the early blastula stage but greatly inhibited by the late blastula (stage 9.5) stage, when the synthesis of DNA and protein was still not inhibited appreciably. Thus, inhibition in RNA synthesis appears to be the first manifestation of the maternal defect that occurs before the gastrulation arrest.
非洲爪蟾（编号65）雌性个体的胚胎在原肠胚形成期停止发育，被认为是卵缺陷突变体。我们将这些胚胎解离，并研究了不同阶段的RNA合成情况。直到原肠胚晚期，卵缺陷胚胎的细胞都像野生型胚胎细胞一样非常活跃地重新聚集。囊胚早期RNA合成正常，但到囊胚晚期（9.5期）受到极大抑制，此时DNA和蛋白质的合成仍未受到明显抑制。因此，RNA合成的抑制似乎是在原肠胚形成期停滞之前出现的母体缺陷的首要表现。
