Furuno N, Shiokawa K, Kobayashi H
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Cell Struct Funct. 1988 Apr;13(2):113-22. doi: 10.1247/csf.13.113.
The amount of intact 40S rRNA precursor was followed by Northern hybridization in the course of the early embryogenesis of wild-type Xenopus laevis and its anucleolate mutant. The total amount of 40S rRNA precursor did not alter appreciably until the midblastula stage, decreased at the late blastula stage, and then increased. In the anucleolate mutant, in which no rRNA synthesis occurs, the 40S rRNA precursor decreased at the late blastula stage and disappeared after the gastrula stage. In the nuclear fraction of the wild type, the 40S rRNA precursor was detectable after the midblastula stage. Therefore, the 40S rRNA precursor in the pre-blastula embryos is maternal and decreases at the late blastula stage. New synthesis of 40S rRNA precursor apparently occurs after the midblastula stage.
在野生型非洲爪蟾及其无核仁突变体的早期胚胎发育过程中，通过Northern杂交追踪完整的40S rRNA前体的量。40S rRNA前体的总量直到囊胚中期都没有明显变化，在囊胚后期减少，然后增加。在不发生rRNA合成的无核仁突变体中，40S rRNA前体在囊胚后期减少，并在原肠胚期后消失。在野生型的细胞核部分，40S rRNA前体在囊胚中期后可检测到。因此，囊胚前期胚胎中的40S rRNA前体是母体来源的，并在囊胚后期减少。40S rRNA前体的新合成显然发生在囊胚中期之后。
