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从食酸丛毛单胞菌BH72中鉴定并生物合成一种新型的野油菜黄单胞菌素-二烷基间苯二酚杂合物。

Identification and biosynthesis of a novel xanthomonadin-dialkylresorcinol-hybrid from Azoarcus sp. BH72.

作者信息

Schöner Tim A, Fuchs Sebastian W, Reinhold-Hurek Barbara, Bode Helge B

机构信息

Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, Frankfurt am Main, Germany.

Lab of General Microbiology, University of Bremen, Bremen, Germany.

出版信息

PLoS One. 2014 Mar 11;9(3):e90922. doi: 10.1371/journal.pone.0090922. eCollection 2014.

DOI:10.1371/journal.pone.0090922
PMID:24618669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3949708/
Abstract

A novel xanthomonadin-dialkylresorcinol hybrid named arcuflavin was identified in Azoarcus sp. BH72 by a combination of feeding experiments, HPLC-MS and MALDI-MS and gene clusters encoding the biosynthesis of this non-isoprenoid aryl-polyene containing pigment are reported. A chorismate-utilizing enzyme from the XanB2-type producing 3- and 4-hydroxybenzoic acid and an AMP-ligase encoded by these gene clusters were characterized, that might perform the first two steps of the polyene biosynthesis. Furthermore, a detailed analysis of the already known or novel biosynthesis gene clusters involved in the biosynthesis of polyene containing pigments like arcuflavin, flexirubin and xanthomonadin revealed the presence of similar gene clusters in a wide range of bacterial taxa, suggesting that polyene and polyene-dialkylresorcinol pigments are more widespread than previously realized.

摘要

通过饲喂实验、高效液相色谱-质谱联用（HPLC-MS）和基质辅助激光解吸电离质谱（MALDI-MS）相结合的方法，在食酸菌属菌株BH72中鉴定出一种新型的黄单胞菌素-二烷基间苯二酚杂合物——弓形黄素，并报道了编码这种含非异戊二烯基芳基多烯色素生物合成的基因簇。对来自XanB2型的利用分支酸产生3-羟基苯甲酸和4-羟基苯甲酸的酶以及这些基因簇编码的AMP连接酶进行了表征，它们可能执行多烯生物合成的前两个步骤。此外，对参与含多烯色素如弓形黄素、弹性红菌素和黄单胞菌素生物合成的已知或新型生物合成基因簇的详细分析表明，在广泛的细菌分类群中存在相似的基因簇，这表明多烯和多烯-二烷基间苯二酚色素比之前认识到的更为广泛。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/1418facb7faf/pone.0090922.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/268b186e2048/pone.0090922.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/9c10c32553be/pone.0090922.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/98d4776da80d/pone.0090922.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/34e4d4e854ec/pone.0090922.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/1418facb7faf/pone.0090922.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/268b186e2048/pone.0090922.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/9c10c32553be/pone.0090922.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/98d4776da80d/pone.0090922.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/34e4d4e854ec/pone.0090922.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f12/3949708/1418facb7faf/pone.0090922.g005.jpg

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