Brunner C, Lassmann H, Waehneldt T V, Matthieu J M, Linington C
Austrian Academy of Sciences and Neurological Institute, University of Vienna.
J Neurochem. 1989 Jan;52(1):296-304. doi: 10.1111/j.1471-4159.1989.tb10930.x.
In a light and electron microscopic immunocytochemical study we have examined the distribution of myelin basic protein (MBP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), and myelin/oligodendroglial glycoprotein (MOG) within CNS myelin sheaths and oligodendrocytes of adult Sprague-Dawley rats. Ultrastructural immunocytochemistry allowed quantitative analysis of antigen density in different myelin and oligodendrocyte zones: MBP was detectable in high density over the whole myelin sheath, but not in regions of loops, somata, or the oligodendrocyte plasma membrane. CNP reactivity was highest at the myelin/axon interface, and found in lower concentration over the outer lamellae of myelin sheaths, at the cytoplasmic face of oligodendrocyte membranes, and throughout the compact myelin. MOG was preferentially detected at the extracellular surface of myelin sheaths and oligodendrocytes and in only low amounts in the lamellae of compacted myelin and the myelin/axon border zone. Our studies, thus, indicate further the presence of different molecular domains in compact myelin, which may be functionally relevant for the integrity and maintenance of the myelin sheath.
在一项光镜和电镜免疫细胞化学研究中，我们检测了成年Sprague-Dawley大鼠中枢神经系统髓鞘和少突胶质细胞中髓鞘碱性蛋白（MBP）、2',3'-环核苷酸3'-磷酸二酯酶（CNP）以及髓鞘/少突胶质细胞糖蛋白（MOG）的分布。超微结构免疫细胞化学能够对不同髓鞘和少突胶质细胞区域的抗原密度进行定量分析：在整个髓鞘上均能检测到高密度的MBP，但在袢、胞体或少突胶质细胞质膜区域未检测到。CNP反应性在髓鞘/轴突界面处最高，在髓鞘外层、少突胶质细胞膜的胞质面以及整个致密髓鞘中浓度较低。MOG主要在髓鞘和少突胶质细胞的细胞外表面检测到，在致密髓鞘板层和髓鞘/轴突边界区域含量较低。因此，我们的研究进一步表明致密髓鞘中存在不同的分子结构域，这可能与髓鞘的完整性和维持在功能上相关。
