Chen Jian-Wen, Zhou Yuan, Xue Zhao-Yun, Li Cheng, Guo Jian, Zhou Li-Yan, Jiang Jian-Min
Zhong Yao Cai. 2013 Sep;36(9):1486-9.
To investigate the effect of Jianpihuashi Decoction on rats with hyperuricemia.
Forty male Sprague-Dawley (SD) rats were randomly divided into four groups: normal, hyperuricemia, Jianpihuashi Decoction and Allopurinol group. After the administration for 0 day, 10 days, 20 days and 30 days, the serum uric acid, creatinine, urea nitrogen and xanthine oxidase (XOD) activity levels were separately detected using the orbital blood. 30 days after the experiment, the rats were anaesthetized by 3% pentobarbital sodium, liver tissue homogenate extracts were used to detect the XOD activity, and histopathological changes in kidney were observed by HE staining.
Treatment with Jianpihuashi Decoction for 30 days, the serum uric acid level of rats with hyperuricemia were significantly decreased (P < 0.05). Simultaneously, the XOD activity in the serum and liver tissue homogenate extracts were obviously decreased by the decoction, which had seldom toxic or side effects on kidney. Allopurinol group could significantly decrease the serum uric acid level, but it had seldom pathological injury to kidney at the same time.
Jianpihuashi Decoction which has seldom pathological injury to kidney can significantly decrease the effect of uric acid by suppressing XOD activity.
探讨健脾化湿汤对高尿酸血症大鼠的影响。
将40只雄性Sprague-Dawley（SD）大鼠随机分为四组：正常组、高尿酸血症组、健脾化湿汤组和别嘌醇组。给药0天、10天、20天和30天后，分别采集眼眶血检测血清尿酸、肌酐、尿素氮及黄嘌呤氧化酶（XOD）活性水平。实验30天后，用3%戊巴比妥钠麻醉大鼠，取肝组织匀浆提取物检测XOD活性，并用苏木精-伊红（HE）染色观察肾脏组织病理学变化。
健脾化湿汤治疗30天后，高尿酸血症大鼠血清尿酸水平显著降低（P<0.05）。同时，该方能明显降低血清及肝组织匀浆提取物中的XOD活性，对肾脏的毒副作用较小。别嘌醇组能显著降低血清尿酸水平，但同时对肾脏的病理损伤较小。
健脾化湿汤对肾脏病理损伤较小，可通过抑制XOD活性显著降低尿酸水平。
