McDaniel L S, Waltman W D, Gray B, Briles D E
Department of Microbiology, University of Alabama, Birmingham 35294.
Microb Pathog. 1987 Oct;3(4):249-60. doi: 10.1016/0882-4010(87)90058-1.
D3114/63 is a hybridoma cell line producing a monoclonal antibody that appears to recognize a common antigenic determinant associated with the teichoic acids of Streptococcus pneumoniae. This hybridoma was produced in a fusion of splenic lymphocytes from an X-linked immunodeficient (xid) male (CBA/NxBALB/c)F1 mouse hyperimmunized with a heat-killed type 6A strain EF-3114. Treatment of EF-3114 with periodate, but not with protease, destroyed its ability to bind D3114/63. The specificity of D3114/63 for teichoic acids was suggested by the ability of both C-polysaccharide and F-antigen to inhibit its binding to EF-3114. Inhibition studies indicated that D3114/63 does not bind the PC determinant of teichoic acids. As in the case with anti-PC antibodies, the presence of an intact pneumococcal capsule significantly reduced the binding of D3114/63 to viable pneumococci. By means of a colony immunoblot procedure, it was determined that D3114/63 bound to all 97 pneumococcal strains tested including representatives of 24 different serotypes. Both D3114/63 and anti-PC antibodies were able to detect pneumococcal antigen in spinal fluids of 3 out of 4 patients with pneumococcal meningitis. D3114/63 protected mice from fatal infection with type 3 strain WU2 pneumococci but not from type 5 strain DBL5 or type 6A strain EF-3114.
D3114/63是一种杂交瘤细胞系，可产生一种单克隆抗体，该抗体似乎能识别与肺炎链球菌磷壁酸相关的共同抗原决定簇。这种杂交瘤是由一只经热灭活的6A 型菌株EF - 3114超免疫的X连锁免疫缺陷（xid）雄性（CBA/N×BALB/c）F1小鼠的脾淋巴细胞融合产生的。用高碘酸盐而非蛋白酶处理EF - 3114，会破坏其与D3114/63结合的能力。C多糖和F抗原均能抑制D3114/63与EF - 3114的结合，这表明D3114/63对磷壁酸具有特异性。抑制研究表明，D3114/63不结合磷壁酸的PC决定簇。与抗PC抗体的情况一样，完整的肺炎球菌荚膜的存在会显著降低D3114/63与活肺炎球菌的结合。通过菌落免疫印迹法确定，D3114/63与所测试的全部97株肺炎球菌菌株结合，包括24种不同血清型的代表菌株。D3114/63和抗PC抗体均能在4例肺炎球菌性脑膜炎患者中的3例患者的脑脊液中检测到肺炎球菌抗原。D3114/63可保护小鼠免受3型菌株WU2肺炎球菌的致命感染，但不能保护其免受5型菌株DBL5或6A型菌株EF - 3114的感染。
