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用于检测尿液样本中淋病奈瑟菌的环介导等温扩增试验及该检测方法对尿素存在的耐受性

Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.

作者信息

Edwards Thomas, Burke Patricia A, Smalley Helen B, Gillies Liz, Hobbs Glyn

机构信息

School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, United Kingdom.

Mast Group Ltd., Bootle, Liverpool, United Kingdom.

出版信息

J Clin Microbiol. 2014 Jun;52(6):2163-5. doi: 10.1128/JCM.00314-14. Epub 2014 Mar 12.

DOI:10.1128/JCM.00314-14
PMID:24622100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4042760/
Abstract

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤ 1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

摘要

一种用于淋病奈瑟菌谷氨酰胺合成酶基因开放阅读框1（ORF1）的环介导等温扩增（LAMP）检测方法能够耐受≤1.8 M的尿素浓度，相比之下，一种聚合酶链反应（PCR）检测方法在浓度<100 mM时才具有功能。LAMP检测方法与PCR检测方法一样灵敏，同时操作更快、更简单。

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