Luijt Dirk S, Bos Petra A J, van Zwet Anton A, van Voorst Vader Pieter C, Schirm Jurjen
Regional Public Health Laboratory, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands.
J Clin Microbiol. 2005 Mar;43(3):1445-7. doi: 10.1128/JCM.43.3.1445-1447.2005.
A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.
使用COBAS AMPLICOR(CA)聚合酶链反应(PCR)对总共3023份临床标本进行淋病奈瑟菌检测,并通过淋病奈瑟菌特异性16S核糖体RNA(rRNA)PCR对阳性结果进行确认。CA加16S rRNA PCR的敏感性为98.8%,而培养法的敏感性为68.2%。对CA阳性结果进行确认使阳性预测值从54.8%提高到96.6%。