Probst Sonja, Wiehr Stefan, Mantlik Frederic, Schmidt Holger, Kolb Armin, Münch Peter, Delcuratolo Maria, Stubenrauch Frank, Pichler Bernd J, Iftner Thomas
Mol Imaging. 2014;13. doi: 10.2310/7290.2013.00070.
In this study, simultaneous positron emission tomography (PET)/magnetic resonance (MR) imaging was employed to evaluate the feasibility of the PET tracers 2-deoxy-2-18F-fluoro-d-glucose (18F-FDG), 11C-choline, and 18F-fluorothymidine (18F-FLT) to detect papillomavirus-induced tumors in an established rabbit model system. The combined PET/MR allowed the analysis of tracer uptake of the tumors using the morphologic information acquired by MR. New Zealand White rabbits were infected with cottontail rabbit papillomavirus genomes and were imaged for up to 10 months with a simultaneous PET/MR system during the course of infection. The uptake characteristics of the PET tracers 11C-choline and 18F-FLT of tumors and reference tissues were examined relative to the clinical standard, 18F-FDG. Tracer biodistribution of various organs was measured by gamma-counting after the last PET scan and compared to the in vivo PET/MR 18F-FDG uptake. Increased tracer uptake was found 2 months postinfection in primary tumors with 18F-FDG and 11C-choline, whereas 18F-FLT failed to detect the tumors at all measured time points. Our data show that the PET tracer 18F-FDG is superior for imaging papillomavirus-induced tumors in rabbits compared to 11C-choline and 18F-FLT. However, 11C-choline imaging, which has previously been applied to detect various tumor entities in patients, appears to be an alternative to 18F-FDG.
在本研究中，采用正电子发射断层扫描（PET）/磁共振（MR）成像技术，评估PET示踪剂2-脱氧-2-¹⁸F-氟-D-葡萄糖（¹⁸F-FDG）、¹¹C-胆碱和¹⁸F-氟胸苷（¹⁸F-FLT）在已建立的兔模型系统中检测乳头瘤病毒诱导肿瘤的可行性。PET/MR联合成像能够利用MR获取的形态学信息分析肿瘤对示踪剂的摄取情况。将新西兰白兔感染棉尾兔乳头瘤病毒基因组，并在感染过程中使用PET/MR同步系统对其进行长达10个月的成像。相对于临床标准¹⁸F-FDG，研究了¹¹C-胆碱和¹⁸F-FLT这两种PET示踪剂在肿瘤及参考组织中的摄取特征。在最后一次PET扫描后，通过γ计数测量各器官的示踪剂生物分布，并与体内PET/MR的¹⁸F-FDG摄取情况进行比较。在感染后2个月，¹⁸F-FDG和¹¹C-胆碱在原发性肿瘤中出现示踪剂摄取增加，而¹⁸F-FLT在所有测量时间点均未能检测到肿瘤。我们的数据表明，与¹¹C-胆碱和¹⁸F-FLT相比，PET示踪剂¹⁸F-FDG在兔乳头瘤病毒诱导肿瘤的成像方面更具优势。然而，此前已应用于检测患者各种肿瘤实体的¹¹C-胆碱成像似乎可作为¹⁸F-FDG的替代方法。
