Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
J Physiol Pharmacol. 2014 Feb;65(1):107-15.
Potential effects of prenatal administration of acrylamide (ACR) on postnatal development of the small intestine were not examined experimentally yet. The aim of this study was to establish changes of morphological parameters of the small intestine damaged by prenatal action of ACR in guinea pigs. The 3 mg/kg body weight of ACR was given in drinking water every day during the last 35 days of the pregnancy in guinea pigs. The histomorphometry of the duodenum and jejunum was determined. Immunohistochemical staining with anti cadherin antibody was performed. Maternal treatment with ACR led to the decrease of the expression of cadherin in the epithelium. Maternal ACR treatment increased the number of total, divided and inactive crypt, and the number of damaged villi in the duodenum and jejunum of newborn guinea pigs. The thickness of myenteron and submucosa, mucosa fractal dimension and the depth of crypts in the duodenum were increased by ACR. Additionally, in offspring born by mothers administered with ACR the decrease of villi epithelium thickness and active crypt number was observed. Moreover, ACR decreased goblet cells and inact villi number in the duodenum, mucosa thickness and crypts width in the jejunum. Intestine absorptive surface was affected by ACR in the jejunum as well. Results of measurements showed that maternal ACR treatment had negative influence on small intestine histomorphometry. ACR acting prenatally influenced small intestine nervous plexuses that became enlarged by 2.5 times compared with the control group. In conclusion, our results showed the negative impact of maternal ACR treatment on histological structure, integrity and innervation of small intestine wall as well as on absorptive function of small intestine mucosa.
目前尚未有实验研究探讨丙烯酰胺（ACR）在产前对小肠发育的潜在影响。本研究旨在确定豚鼠产前 ACR 作用对小肠形态学参数的损伤变化。在妊娠的最后 35 天，豚鼠通过饮用含 3mg/kg 体重 ACR 的水来进行每日处理。对十二指肠和空肠进行组织形态计量学测定，并用抗钙粘蛋白抗体进行免疫组织化学染色。母体 ACR 处理导致上皮钙粘蛋白表达减少。母体 ACR 处理增加了十二指肠和空肠中总、分裂和无活性隐窝的数量，以及受损绒毛的数量。 ACR 增加了肌间神经丛和黏膜下层、黏膜分形维数和十二指肠隐窝深度的厚度。此外，在接受 ACR 处理的母亲所生的后代中，观察到绒毛上皮厚度和活跃隐窝数量减少。此外，ACR 减少了十二指肠中的杯状细胞和无活性绒毛的数量、空肠的黏膜厚度和隐窝宽度。ACR 还影响了空肠的小肠吸收表面。测量结果表明，母体 ACR 处理对小肠组织形态学有负面影响。产前 ACR 作用影响了小肠神经丛，使其与对照组相比扩大了 2.5 倍。总之，我们的研究结果表明，母体 ACR 处理对小肠壁的组织学结构、完整性和神经支配以及小肠黏膜的吸收功能都有负面影响。
