Corrales Isabel, Giménez Estela, Navarro David
Microbiology Service, University Clinic Hospital Institute for Clinical Research (INCLIVA), Valencia, Spain.
Clin Vaccine Immunol. 2014 May;21(5):684-8. doi: 10.1128/CVI.00104-14. Epub 2014 Mar 12.
Commercial immunoassays for detecting IgG and IgM antibodies against Epstein-Barr virus (EBV), viral capsid antigens (VCA), and IgGs toward EBV nuclear antigen-1 (EBNA-1) are routinely used in combination to categorize EBV infection status. In this study, we evaluated the performances of the Architect EBV VCA IgG, VCA IgM, and EBNA-1 IgG chemiluminescent microparticle assays (CMIAs) in EBV serological analyses using indirect immunofluorescence assays and anticomplement immunofluorescence assays as the reference methods for VCA IgG, VCA IgM, and EBNA-1 IgG antibody detection, respectively. A total of 365 serum samples representing different EBV serological profiles were included in this study. The κ values (concordances between the results) obtained in the Architect CMIA and those in the reference assays were 0.905 (P < 0.0001) for VCA IgM, 0.889 (P < 0.0001) for VCA IgG, and 0.961 (P < 0.0001) for EBNA-1 IgG. The sensitivities and specificities were, respectively, 91.08% and 99.48% for VCA IgM, 99.23% and 86.27% for VCA IgG, and 96.77% and 99.16% for EBNA-1 IgG. The sensitivities and specificities of the Architect CMIA panel were, respectively, 99.15% and 98.6% for diagnosing a primary infection, 97.62% and 93.39% for diagnosing a past EBV infection, and 92.42% and 97.82% for diagnosing the absence of an EBV infection. In summary, we demonstrated that the Architect EBV antibody panel performs very well for EBV antibody detection and correctly categorizes clinically relevant EBV infection states.
用于检测抗EB病毒（EBV）的IgG和IgM抗体、病毒衣壳抗原（VCA）以及抗EBV核抗原1（EBNA-1）的IgG的商业免疫测定法通常联合使用，以对EBV感染状态进行分类。在本研究中，我们使用间接免疫荧光测定法和抗补体免疫荧光测定法分别作为VCA IgG、VCA IgM和EBNA-1 IgG抗体检测的参考方法，评估了Architect EBV VCA IgG、VCA IgM和EBNA-1 IgG化学发光微粒子免疫测定法（CMIA）在EBV血清学分析中的性能。本研究共纳入了365份代表不同EBV血清学谱的血清样本。在Architect CMIA与参考测定法之间获得的κ值（结果之间的一致性），VCA IgM为0.905（P < 0.0001），VCA IgG为0.889（P < 0.0001），EBNA-1 IgG为0.961（P < 0.0001）。VCA IgM的敏感性和特异性分别为91.08%和99.48%，VCA IgG为99.23%和86.27%，EBNA-1 IgG为96.77%和99.16%。Architect CMIA检测组诊断原发性感染的敏感性和特异性分别为99.15%和98.6%，诊断既往EBV感染为97.62%和93.39%，诊断无EBV感染为92.42%和97.82%。总之，我们证明了Architect EBV抗体检测组在EBV抗体检测方面表现出色，并能正确分类临床相关的EBV感染状态。
