Lee Hye Ryun, Shin Sue, Yoon Jong Hyun, Roh Eun Youn, Kim Byoung Jae, Song Eun Young
Department of Laboratory Medicine, Gyeongsang National University Hospital, Jinju, Korea.
Transfusion. 2014 Jul;54(7):1871-5. doi: 10.1111/trf.12555. Epub 2014 Mar 14.
The number of aldehyde dehydrogenase-bright (ALDH(br) ) cells has been suggested as a viable marker of hematopoietic stem cell function. We evaluated the suitability of ALDH(br) cell analysis in the quality assessment of postthaw cord blood (CB) units.
A total of 245 CB units were obtained for estimating the numbers of total nucleated cells (TNCs), CD34+ cells, ALDH(br) cells, ALDH(br) cells among CD34+ cells (CD34+ALDH(br) cells), CD34+ cells among ALDH(br) cells (ALDH(br) CD34+ cells), colony-forming unit (CFU)-granulocyte-macrophages (GMs), and CFU-granulocyte-erythrocyte-macrophage-megakaryocytes (GEMMs). Simple linear regression analysis was performed to assess the correlation between the number of TNCs and CD34+ cells before and after crypreservation and CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells after cryopreservation and the number of CFU-GEMMS and CFU-GMs.
The number of CFU-GMs was found to be significantly correlated with the number of CD34+ cells before and after cryopreservation (r = 0.418 and r = 0.359, respectively), CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells (r = 0.426, r = 0.455, and r = 0.469, respectively). The number of CFU-GEMMs was found to be significantly correlated with the number of TNCs and CD34+ cells before and after cryopreservation (TNCs, r = 0.251 and r = 0.250, respectively; CD34+ cells, r = 0.391 and r = 0.347, respectively), CD34+ALDH(br) cells, ALDH(br) cells, and ALDH(br) CD34+ cells (r = 0.297, r = 0.297, and r = 0.252, respectively).
The high correlation found between ALDH activity and CFU-GM number supports the suitability of ALDH analysis in the quality assessment of postthaw CB units.
醛脱氢酶高表达（ALDH(br)）细胞数量已被认为是造血干细胞功能的一个可行标志物。我们评估了ALDH(br)细胞分析在解冻后脐带血（CB）单位质量评估中的适用性。
共获取245份CB单位样本，用于估计总核细胞（TNC）、CD34+细胞、ALDH(br)细胞、CD34+细胞中的ALDH(br)细胞（CD34+ALDH(br)细胞）、ALDH(br)细胞中的CD34+细胞（ALDH(br)CD34+细胞）、集落形成单位（CFU）-粒细胞-巨噬细胞（GM）和CFU-粒细胞-红细胞-巨噬细胞-巨核细胞（GEMM）的数量。进行简单线性回归分析，以评估冷冻保存前后TNC和CD34+细胞数量与冷冻保存后CD34+ALDH(br)细胞、ALDH(br)细胞和ALDH(br)CD34+细胞数量以及CFU-GEMM和CFU-GM数量之间的相关性。
发现CFU-GM数量与冷冻保存前后CD34+细胞数量显著相关（分别为r = 0.418和r = 0.359），与CD34+ALDH(br)细胞、ALDH(br)细胞和ALDH(br)CD34+细胞数量也显著相关（分别为r = 0.426、r = 0.455和r = 0.469）。发现CFU-GEMM数量与冷冻保存前后TNC和CD34+细胞数量显著相关（TNC，分别为r = 0.251和r = 0.250；CD34+细胞，分别为r = 0.391和r = 0.347），与CD34+ALDH(br)细胞、ALDH(br)细胞和ALDH(br)CD34+细胞数量也显著相关（分别为r = 0.297、r = 0.297和r = 0.252）。
ALDH活性与CFU-GM数量之间的高度相关性支持了ALDH分析在解冻后CB单位质量评估中的适用性。
