Varma V A, Austin G E, O'Connell A C
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, GA.
Arch Pathol Lab Med. 1989 Jan;113(1):16-9.
Increased expression of ras oncogene protein p21 has been claimed to be associated with neoplastic transformation in histochemical studies of prostate and other tissues. Therefore, we evaluated the expression of p21 in formaldehyde-fixed, paraffin-embedded prostatic tissue using a commercially available polyclonal antiserum prepared in sheep. We found no consistent difference in p21 expression between benign and malignant epithelium and no correlation between tumor grade and intensity of p21 staining. Our results do not support the contention that neoplastic transformation of human prostate is associated with a histochemically demonstrable increase in p21 expression.
在前列腺及其他组织的组织化学研究中，有人声称癌基因蛋白p21表达增加与肿瘤转化有关。因此，我们使用市售的羊制备的多克隆抗血清，评估了甲醛固定、石蜡包埋前列腺组织中p21的表达。我们发现良性和恶性上皮之间p21表达没有一致差异，肿瘤分级与p21染色强度之间也没有相关性。我们的结果不支持人类前列腺肿瘤转化与p21表达在组织化学上可证实的增加有关的论点。
