Panjehshahin M R, Bowmer C J, Yates M S
Department of Pharmacology, University of Leeds, U.K.
Biochem Pharmacol. 1989 Jan 1;38(1):155-9. doi: 10.1016/0006-2952(89)90162-7.
Double-reciprocal plots of fluorescence intensity versus protein concentration are often used to obtain the intrinsic molar fluorescence (Fb) of ligands bound to acceptor molecules such as albumin. In this paper we show that these plots develop upward concave curvature as the concentration of albumin increases. Thus linear extrapolation of such plots cannot be employed to provide accurate values of Fb. It is suggested that a direct plot of fluorescence intensity versus log protein concentration should be employed to obtain Fb.
荧光强度与蛋白质浓度的双倒数图常用于获得与诸如白蛋白等受体分子结合的配体的固有摩尔荧光（Fb）。在本文中，我们表明随着白蛋白浓度的增加，这些图会出现向上的凹曲率。因此，不能采用此类图的线性外推来提供Fb的准确值。建议采用荧光强度与蛋白质浓度对数的直接图来获得Fb。
