Bech Rikke, Otkjaer Kristian, Birkelund Svend, Vorup-Jensen Thomas, Agger Ralf, Johansen Claus, Iversen Lars, Kragballe Knud, Rømer John
Department of Dermatology, Aarhus University Hospital, Aarhus, Denmark; Department of Biomedicine, Aarhus University, Aarhus, Denmark.
Exp Dermatol. 2014 May;23(5):349-52. doi: 10.1111/exd.12371.
We previously demonstrated that mRNA for the pro-inflammatory cytokine interleukin 20 (IL-20) is expressed in suprapapillary keratinocytes of lesional psoriatic skin (LS). Here, we describe the distribution of IL-20 protein and the identity of the IL-20-positive cells in LS. We found that the main part of IL-20 immunoreactivity is present in mononuclear cells of the dermal papillae, and that the IL-20-positive cells located in the papillae were langerin+, CD1a+, CD4+ and CD303+. These cells might be immature dendritic cell. In situ hybridization for IL-20 mRNA on non-LS, ex vivo stimulated with IL-1β revealed a colocalization between IL-20 mRNA and the keratinocyte marker CK14. No IL-20 mRNA was detected in the dermal mononuclear cells. Our results suggest that IL-20 is produced by keratinocytes, released into the epidermis and then possibly taken up by papillary mononuclear cells. Our study supports that IL-20 is involved in the pathogenesis of psoriasis.
我们之前证明，促炎细胞因子白细胞介素20（IL-20）的mRNA在银屑病皮损（LS）的乳头上方角质形成细胞中表达。在此，我们描述了IL-20蛋白在LS中的分布以及IL-20阳性细胞的特征。我们发现，IL-20免疫反应性的主要部分存在于真皮乳头的单核细胞中，且位于乳头中的IL-20阳性细胞为朗格汉斯蛋白阳性、CD1a阳性、CD4阳性和CD303阳性。这些细胞可能是未成熟的树突状细胞。对非LS进行IL-1β体外刺激后，针对IL-20 mRNA的原位杂交显示IL-20 mRNA与角质形成细胞标志物CK14共定位。在真皮单核细胞中未检测到IL-20 mRNA。我们的结果表明，IL-20由角质形成细胞产生，释放到表皮中，然后可能被乳头单核细胞摄取。我们的研究支持IL-20参与银屑病的发病机制。
