Grossman R M, Krueger J, Yourish D, Granelli-Piperno A, Murphy D P, May L T, Kupper T S, Sehgal P B, Gottlieb A B
Rockefeller University, New York, NY 10021-6399.
Proc Natl Acad Sci U S A. 1989 Aug;86(16):6367-71. doi: 10.1073/pnas.86.16.6367.
Psoriasis is a common papulosquamous skin disease. The histopathology is characterized by epidermal hyperplasia and inflammation. Recent studies suggest that keratinocyte proliferation and inflammation in psoriasis are manifestations of the same underlying pathological process. Interleukin 6 (IL-6), a cytokine that is a major mediator of the host response to tissue injury and infection, is produced by both keratinocytes and leukocytes in culture. IL-6 expression was studied in psoriatic plaques by immunoperoxidase staining with two different polyclonal anti-recombinant IL-6 antisera and by in situ nucleic acid hybridization with IL-6 cRNA probes. Epidermal and dermal cells in active psoriatic plaques from 35 psoriasis patients stained heavily for IL-6 as compared with nonlesional skin and with plaques after treatment with antimetabolic and antiinflammatory agents. Absorption of the anti-recombinant IL-6 antisera with purified fibroblast-derived IL-6 or with recombinant IL-6, but not bovine serum albumin, removed the immunostaining. Increased levels of IL-6 were detected in the plasma of patients with active psoriasis (mean 3 ng/ml) by using two different bioassays. IL-6 production by proliferating keratinocytes was suggested by IL-6-specific immunostaining in cultured normal and psoriatic keratinocytes and by the detection of mRNA specific for IL-6 in psoriatic epidermis by in situ hybridization. IL-6 stimulated the proliferation of cultured, normal human keratinocytes as assessed by two different assays. Thus, IL-6 could directly contribute to the epidermal hyperplasia seen in psoriatic epithelium as well as affect the function of dermal inflammatory cells.
银屑病是一种常见的丘疹鳞屑性皮肤病。其组织病理学特征为表皮增生和炎症。最近的研究表明，银屑病中的角质形成细胞增殖和炎症是同一潜在病理过程的表现。白细胞介素6（IL-6）是一种细胞因子，是宿主对组织损伤和感染反应的主要介质，在培养的角质形成细胞和白细胞中均可产生。通过用两种不同的多克隆抗重组IL-6抗血清进行免疫过氧化物酶染色以及用IL-6 cRNA探针进行原位核酸杂交，对银屑病斑块中的IL-6表达进行了研究。与非皮损皮肤以及用抗代谢药和抗炎药治疗后的斑块相比，35例银屑病患者的活动性银屑病斑块中的表皮和真皮细胞IL-6染色强烈。用纯化的成纤维细胞衍生的IL-6或重组IL-6而非牛血清白蛋白吸收抗重组IL-6抗血清后，免疫染色消失。通过两种不同的生物测定法检测到活动性银屑病患者血浆中IL-6水平升高（平均3 ng/ml）。培养的正常和银屑病角质形成细胞中的IL-6特异性免疫染色以及原位杂交检测银屑病表皮中IL-6特异性mRNA，提示增殖的角质形成细胞产生IL-6。通过两种不同的测定法评估，IL-6刺激了培养的正常人角质形成细胞的增殖。因此，IL-6可能直接导致银屑病上皮中所见的表皮增生，并影响真皮炎症细胞的功能。
