四膜虫纤毛在K⁺和Ca²⁺混合溶液中的吉布斯-唐南比率与通道电导
Gibbs-Donnan ratio and channel conductance of Tetrahymena cilia in mixed solution of K+ and Ca2+.
作者信息
Oosawa Y, Kasai M
机构信息
Department of Biophysical Engineering, Faculty of Engineering Sciences, Osaka University, Japan.
出版信息
Biophys J. 1988 Sep;54(3):407-10. doi: 10.1016/S0006-3495(88)82974-6.
Abstract
A single cation-channel from Tetrahymena cilia was incorporated into planar lipid bilayers. This channel was voltage-independent and is permeable to K+ and Ca2+. In the experiments with mixed solutions where the concentrations of K+ and Ca2+ were varied, the single-channel conductance was found to be influenced by the Gibbs-Donnan ratio. The data are explained by assuming that the binding sites of this channel were always occupied by two potassium ions or one calcium ion under the present experimental conditions (5 mM-90 mM K+ and 0.5 mM-35 mM Ca2+) and these bound cations determined the channel conductivity.
摘要
来自四膜虫纤毛的单个阳离子通道被整合到平面脂质双分子层中。该通道不依赖电压，对K⁺和Ca²⁺具有通透性。在K⁺和Ca²⁺浓度变化的混合溶液实验中，发现单通道电导受吉布斯-唐南比率影响。通过假设在当前实验条件下（5 mM - 90 mM K⁺和0.5 mM - 35 mM Ca²⁺）该通道的结合位点总是被两个钾离子或一个钙离子占据，且这些结合的阳离子决定通道电导率，来解释这些数据。
相似文献
1
Gibbs-Donnan ratio and channel conductance of Tetrahymena cilia in mixed solution of K+ and Ca2+.四膜虫纤毛在K⁺和Ca²⁺混合溶液中的吉布斯-唐南比率与通道电导
Biophys J. 1988 Sep;54(3):407-10. doi: 10.1016/S0006-3495(88)82974-6.
2
Ionic currents of channels that are permeable to monovalent and divalent cations.对单价和二价阳离子具有通透性的通道的离子电流。
Biophys J. 1989 Dec;56(6):1217-23. doi: 10.1016/S0006-3495(89)82768-7.
3
Cation channels from Tetrahymena cilia incorporated into planar lipid bilayers.
Am J Physiol. 1985 Jul;249(1 Pt 1):C177-9. doi: 10.1152/ajpcell.1985.249.1.C177.
4
A cation channel for K+ and Ca2+ from Tetrahymena cilia in planar lipid bilayers.
Cell Struct Funct. 1988 Feb;13(1):51-60. doi: 10.1247/csf.13.51.
5
Ion permeability of ciliary membrane vesicles isolated from Tetrahymena. Single-channel recording study on the membrane reconstituted into a planar lipid bilayer.
J Biochem. 1986 Dec;100(6):1569-73. doi: 10.1093/oxfordjournals.jbchem.a121864.
6
Ion channels permeable to monovalent and divalent cations: a single-file two-site channel model.对单价和二价阳离子通透的离子通道：单排双位点通道模型
Jpn J Physiol. 2001 Oct;51(5):569-76. doi: 10.2170/jjphysiol.51.569.
7
Ion channels in human endothelial cells.人类内皮细胞中的离子通道。
Gen Physiol Biophys. 1990 Apr;9(2):89-111.
8
Reconstitution in planar lipid bilayers of a Ca2+-dependent K+ channel from transverse tubule membranes isolated from rabbit skeletal muscle.从兔骨骼肌分离的横小管膜中Ca2+依赖性K+通道在平面脂质双分子层中的重组。
Proc Natl Acad Sci U S A. 1982 Feb;79(3):805-9. doi: 10.1073/pnas.79.3.805.
9
Voltage-dependent calcium channels from brain incorporated into planar lipid bilayers.来自大脑的电压依赖性钙通道整合到平面脂质双分子层中。
Nature. 1984;308(5954):77-80. doi: 10.1038/308077a0.
10
A high conductance cationic channel from Phaseolus vulgaris roots incorporated into planar lipid bilayers.来自菜豆根的一种高电导阳离子通道整合到平面脂质双分子层中。
Arch Biochem Biophys. 2005 Jun 1;438(1):88-92. doi: 10.1016/j.abb.2005.04.007. Epub 2005 Apr 26.
引用本文的文献
1
Calcium-dependent potassium channel in Paramecium studied under patch clamp.
J Membr Biol. 1989 Nov;112(1):79-89. doi: 10.1007/BF01871166.
2
Ionic currents of channels that are permeable to monovalent and divalent cations.对单价和二价阳离子具有通透性的通道的离子电流。
Biophys J. 1989 Dec;56(6):1217-23. doi: 10.1016/S0006-3495(89)82768-7.
3
Ca(2+)-activated K+ channels from an insulin-secreting cell line incorporated into planar lipid bilayers.来自胰岛素分泌细胞系的钙激活钾通道整合到平面脂质双分子层中。
Diabetologia. 1992 Jul;35(7):619-23. doi: 10.1007/BF00400252.
本文引用的文献
1
The mechanism of ciliary movement. II. Ion antagonism and ciliary reversal.纤毛运动的机制。II. 离子拮抗作用与纤毛反转
J Cell Comp Physiol. 1962 Dec;60:217-28. doi: 10.1002/jcp.1030600305.
2
Biochemical studies of the excitable membrane of Paramecium tetraurelia. III. Proteins of cilia and ciliary membranes.四膜虫可兴奋膜的生化研究。III. 纤毛和纤毛膜的蛋白质
J Cell Biol. 1980 Mar;84(3):717-38. doi: 10.1083/jcb.84.3.717.
3
Mechanism of ion permeation through calcium channels.离子通过钙通道的渗透机制。
Nature. 1984;309(5967):453-6. doi: 10.1038/309453a0.
4
The physiological basis of taxes in Paramecium.草履虫趋性的生理基础。
Annu Rev Physiol. 1982;44:519-34. doi: 10.1146/annurev.ph.44.030182.002511.
5
Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore.蛙肌钙通道的非选择性电导：单排孔道中的钙选择性
J Physiol. 1984 Aug;353:585-608. doi: 10.1113/jphysiol.1984.sp015352.
6
Binding of Ca ions by Paramecium caudatum.尾草履虫对钙离子的结合作用。
J Gen Physiol. 1967 May;50(5):1303-10. doi: 10.1085/jgp.50.5.1303.
7
Experimental and theoretical studies on Tl+ interactions with the cation-selective channel of the sarcoplasmic reticulum.铊离子（Tl+）与肌浆网阳离子选择性通道相互作用的实验与理论研究
J Membr Biol. 1985;84(1):9-23. doi: 10.1007/BF01871644.
8
Monovalent and divalent cation permeation in acetylcholine receptor channels. Ion transport related to structure.乙酰胆碱受体通道中的单价和二价阳离子渗透。与结构相关的离子转运。
J Gen Physiol. 1987 Jun;89(6):959-83. doi: 10.1085/jgp.89.6.959.
9
Monovalent ion current through single calcium channels of skeletal muscle transverse tubules.通过骨骼肌横管单钙通道的单价离子电流。
Biophys J. 1987 Mar;51(3):497-502. doi: 10.1016/S0006-3495(87)83371-4.
10
Calcium channel selectivity for divalent and monovalent cations. Voltage and concentration dependence of single channel current in ventricular heart cells.钙通道对二价和一价阳离子的选择性。心室肌细胞单通道电流的电压和浓度依赖性。
J Gen Physiol. 1986 Sep;88(3):293-319. doi: 10.1085/jgp.88.3.293.
Zotero 插件