Naitoh Y, Yasumasu I
J Gen Physiol. 1967 May;50(5):1303-10. doi: 10.1085/jgp.50.5.1303.
Binding of (45)Ca by live Paramecium caudatum was determined under various external ionic conditions. It was found that calcium uptake was separable into at least two components, a rapid and a slow one. The rapid component was influenced by the presence of certain other ions in a manner which agrees with the law of mass action. It appears that an ion exchange system may be involved in a binding equilibrium established between Paramecium, Ca(++), and certain other ions. K(+), Rb(+), and Ba(++) in the equilibrium medium are among those ions which inhibit calcium uptake. It is proposed that liberation of Ca(++) from binding sites on Paramecium by an exchange reaction with competing ions is the first step in the mechanism of ciliary reversal in the response to external application of these ions.
在不同的外部离子条件下测定了活尾草履虫对(45)钙的结合情况。发现钙的摄取至少可分为两个部分,一个快速部分和一个缓慢部分。快速部分受某些其他离子存在的影响,其方式符合质量作用定律。看来离子交换系统可能参与了草履虫、钙离子和某些其他离子之间建立的结合平衡。平衡介质中的钾离子、铷离子和钡离子是抑制钙摄取的离子。有人提出,通过与竞争离子的交换反应使钙离子从草履虫上的结合位点释放出来,是这些离子外部施加时纤毛逆转机制的第一步。