PDZ 结合激酶/T-LAK 细胞起源的蛋白激酶（PBK/TOPK）在人膀胱移行细胞癌中的表达。
Expression of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) in human urinary bladder transitional cell carcinoma.
机构信息
Department of Radiotherapy, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
Department of Urology, King George's Medical University, Lucknow 226003, Uttar Pradesh, India.
出版信息
Immunobiology. 2014 Jun;219(6):469-74. doi: 10.1016/j.imbio.2014.02.003. Epub 2014 Feb 25.
The objective of this study was to evaluate the expression pattern of PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) and its clinical significance in human bladder cancer (BC). We detected PBK/TOPK mRNA overexpression in BC and human normal testis tissues using RT-PCR. Using qRT-PCR revealed a higher expression of PBK/TOPK in BC tissues than their adjacent noncancerous tissues (ANCTs) (p<0.0001). Cytoplasmic expression of PBK/TOPK protein was found to be positive in 64.6% (42 of 65) BC patients. Expression of PBK/TOPK protein was found to be significantly higher in muscle-invasive bladder cancer (MIBC) than in non-muscle-invasive bladder cancer (NMIBC) (86.1% vs. 37.9%, p<0.001). The immunohistochemical (IHC) expression of PBK/TOPK was found to be significantly (p<0.001) associated with the stage of disease. Study findings suggest that the PBK/TOPK mRNA/protein expression is specific to human BC and might be used as a novel target for development of cancer immunotherapy and diagnostic biomarker.
本研究旨在评估 PDZ 结合激酶/T-LAK 细胞起源的蛋白激酶（PBK/TOPK）在膀胱癌（BC）中的表达模式及其临床意义。我们使用 RT-PCR 检测了 BC 和人正常睾丸组织中 PBK/TOPK mRNA 的过表达。qRT-PCR 显示 PBK/TOPK 在 BC 组织中的表达高于其相邻非癌组织（ANCT）（p<0.0001）。在 64.6%（65 例中的 42 例）BC 患者中发现 PBK/TOPK 蛋白的细胞质表达为阳性。在肌层浸润性膀胱癌（MIBC）中，PBK/TOPK 蛋白的表达明显高于非肌层浸润性膀胱癌（NMIBC）（86.1%比 37.9%，p<0.001）。免疫组织化学（IHC）表达研究发现，PBK/TOPK 的表达与疾病的分期显著相关（p<0.001）。研究结果表明，PBK/TOPK mRNA/蛋白的表达是 BC 的特异性标志物，可能作为癌症免疫治疗和诊断生物标志物的新靶点。
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