QOPNA, Chemistry Department, University of Aveiro, 3810-193 Aveiro, Portugal.
Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal.
Food Chem. 2014 Aug 1;156:204-11. doi: 10.1016/j.foodchem.2014.01.106. Epub 2014 Feb 7.
In this study, the antiradical and antiproliferative effects of the sesquiterpenic compounds trans, trans-farnesol, cis-nerolidol, α-humulene and guaiazulene, commonly found in plants and plant-derived foods and beverages, were evaluated. Chemical (DPPH and hydroxyl radicals) and biological (Caco-2 cells) models were used. Guaiazulene (IC50=0.73mM) showed higher scavenger capacity against DPPH, while trans, trans-farnesol (IC50=1.81mM) and cis-nerolidol (IC50=1.48mM) were more active towards hydroxyl radicals. All compounds, with the exception of α-humulene, were able to protect Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide. As antiproliferative agents, guaiazulene and cis-nerolidol were more effective than trans, trans-farnesol and α-humulene. The results obtained for the sesquiterpenic compounds by these in vitro assays opens a perspective for their promising use as antioxidants and antiproliferative agents. However, in vivo tests should be carried out in the future to confirm their safety and effectiveness.
在这项研究中，评估了植物和植物源性食品和饮料中常见的倍半萜类化合物反式，反式法呢醇、顺式橙花叔醇、α-葎草烯和玉红草烯的抗氧化和抗增殖作用。使用了化学（DPPH 和羟基自由基）和生物（Caco-2 细胞）模型。玉红草烯（IC50=0.73mM）对 DPPH 的清除能力较高，而反式，反式法呢醇（IC50=1.81mM）和顺式橙花叔醇（IC50=1.48mM）对羟基自由基更活跃。除α-葎草烯外，所有化合物都能够保护 Caco-2 细胞免受叔丁基过氧化物诱导的氧化应激。作为增殖抑制剂，玉红草烯和顺式橙花叔醇比反式，反式法呢醇和α-葎草烯更有效。这些体外试验对倍半萜类化合物的结果为它们作为抗氧化剂和增殖抑制剂的有前途的应用开辟了前景。然而，未来应进行体内试验以确认其安全性和有效性。
