Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados UFGD, MS, Brazil.
Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil.
J Ethnopharmacol. 2018 Jan 10;210:351-358. doi: 10.1016/j.jep.2017.08.030. Epub 2017 Aug 24.
Leaves from Psidium guineense Sw. are used in popular medicine for the treatment of inflammatory disease. However, there is no scientific evidence demonstrating this activity.
To evaluate the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of P. guineense and spathulenol (a major constituent). The study was conducted in part to provide evidence supporting the ethnobotanical use of the leaves of this species.
The essential oil (EOPG) was extracted from the leaves of P. guineense by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). The major compound, spathulenol (PG-1), was isolated in a chromatographic column and characterized by nuclear magnetic resonance (NMR). EOPG and PG-1 were evaluated in vitro for antioxidant activity by DPPH, ABTS and MDA methods; anti-inflammatory potential was assessed using two models, including pleurisy and oedema, in mice. The impact of EOPG and PG-1 on cell proliferation was determined via spectrophotometric quantification of the cellular protein content using a sulforhodamine B assay, and anti-Mycobacterium tuberculosis activity was determined using the REMA method.
A total of 38 components were identified from the EOPG, with the sesquiterpenic alcohol spathulenol (PG-1) (80.7%) being the major constituent. EOPG and PG-1 exhibited the highest antioxidant activities in the DPPH and MDA system compared with reference standard, with IC values ranging from 26.13 to 85.60μg/mL. Oral administration of EOPG and PG-1 showed significant inhibition in the Cg-induced mice paw oedema and pleurisy model. The EOPG (GI = 0.89μg/mL) and PG-1 (GI = 49.30μg/mL) were particularly effective against the ovarian cancer cell line. Both showed moderate antimycobacterial activity.
For the first time, this study demonstrated the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial properties of the essential oil of P. guineense (leaves were collected in Dourados-MS) and spathulenol, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
Psidium guineense Sw. 的叶子被民间医学用于治疗炎症性疾病。然而，目前尚无科学证据证明这种活性。
评估 Psidium guineense 叶子的精油和 spathulenol（主要成分）的抗氧化、抗炎、抗增殖和抗分枝杆菌活性。这项研究旨在为该物种叶子的民族植物学用途提供证据支持。
通过水蒸馏从 Psidium guineense 的叶子中提取精油（EOPG），并通过气相色谱-质谱联用仪（GC-MS）进行分析。主要化合物 spathulenol（PG-1）在色谱柱中分离并通过核磁共振（NMR）进行表征。EOPG 和 PG-1 通过 DPPH、ABTS 和 MDA 方法评估体外抗氧化活性；通过两种模型（包括胸膜炎和水肿）评估抗炎潜力，在小鼠中进行。通过使用 sulforhodamine B 测定法分光光度法定量细胞蛋白含量来确定 EOPG 和 PG-1 对细胞增殖的影响，并且使用 REMA 方法测定抗结核分枝杆菌活性。
从 EOPG 中鉴定出 38 种成分，其中倍半萜醇 spathulenol（PG-1）（80.7%）是主要成分。EOPG 和 PG-1 在 DPPH 和 MDA 系统中表现出最高的抗氧化活性，与参考标准相比，IC 值范围为 26.13 至 85.60μg/mL。EOPG 和 PG-1 口服给药可显著抑制 Cg 诱导的小鼠爪水肿和胸膜炎模型中的爪水肿和胸膜炎。EOPG（GI = 0.89μg/mL）和 PG-1（GI = 49.30μg/mL）对卵巢癌细胞系特别有效。两者均显示出中等的抗分枝杆菌活性。
这项研究首次证明了 Psidium guineense（在 Dourados-MS 收集叶子）精油和 spathulenol 的抗氧化、抗炎、抗增殖和抗分枝杆菌特性，由于其抗炎作用，证实了该植物的民族药理学用途。
