College of Health and Biomedicine, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia.
College of Engineering and Science, Victoria University, Werribee Campus, PO Box 14428, Melbourne, Victoria 8001, Australia.
Food Chem. 2014 Aug 1;156:264-70. doi: 10.1016/j.foodchem.2014.01.105. Epub 2014 Feb 7.
Search for bioactive peptides is intensifying because of the risks associated with the use of synthetic therapeutics, thus peptide liberation by lactic acid bacteria and probiotics has received a great focus. However, proteolytic capacity of these bacteria is strain specific. The study was conducted to establish proteolytic activity of Lactobacillus acidophilus (ATCC® 4356™), Lactobacillus casei (ATCC® 393™) and Lactobacillus paracasei subsp. paracasei (ATCC® BAA52™) in yogurt. Crude peptides were separated by high-speed centrifugation and tested for antioxidant and antimutagenic activities. The degree of proteolysis highly correlated with these bioactivities, and its value (11.91%) for samples containing all the cultures was double that of the control. Liberated peptides showed high radical scavenging activities with 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), IC50 1.51 and 1.63mg/ml, respectively and strong antimutagenicity (26.35%). These probiotics enhanced the generation of bioactive peptides and could possibly be commercially applied in new products, or production of novel anticancer peptides.
由于与合成治疗剂相关的风险，对生物活性肽的搜索正在加剧，因此乳酸菌和益生菌的肽释放受到了极大关注。然而，这些细菌的蛋白水解能力是菌株特异性的。本研究旨在确定嗜酸乳杆菌（ATCC®4356™）、干酪乳杆菌（ATCC®393™）和副干酪乳杆菌亚种副干酪乳杆菌（ATCC®BAA52™）在酸奶中的蛋白水解活性。粗肽通过高速离心分离，并测试其抗氧化和抗诱变活性。酶解程度与这些生物活性高度相关，含有所有培养物的样品的酶解值（11.91%）是对照的两倍。释放的肽对 1,1-二苯基-2-苦基肼基（1,1-diphenyl-2-picrylhydrazyl）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)）具有高自由基清除活性，IC50 分别为 1.51 和 1.63mg/ml，并且具有很强的抗诱变活性（26.35%）。这些益生菌增强了生物活性肽的产生，并且可以在新产品或新型抗癌肽的生产中得到商业应用。
